Pectic acid (Methyl protopectin) 是一种聚半乳糖醛酸，可诱导垂体肿瘤细胞凋亡 (apoptosis) 和坏死 (necrosis)。Pectic acid 可用于癌症和自身免疫性疾病的研究。
| 生物活性 | Pectic acid (Methyl protopectin), a polygalacturonic acid, induces cellapoptosisandnecrosisin pituitary tumor cells. Pectic acid can be used in the research of cancers and autoimmune disease[2][3]. |
| IC50& Target | Human Endogenous Metabolite |
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体外研究
(In Vitro) | Pectic acid (2.5-100 μg/mL, 30 min) stimulates the release of prolactin (PRL) in GH3/B6 cells, without affecting the viability of cells[1].
Pectic acid (100 μg/mL-5 mg/mL, 6-48 h) increases cell death and DNA damage in GH3/B6 cells, detected by MTT assay and AO/EB staining[2].
Pectic acid (100 μg/mL-1 mg/mL, 24 h) induces apoptosis in GH3/B6 cells in a dose-dependent manner[2].
Pectic acid (2.5-5 mg/mL, 24 h) induces necrosis in GH3/B6 cells, confirmed by PI staining[2].
Cell Cycle Analysis[2] | Cell Line: | GH3/B6 cells | | Concentration: | 1 mg/mL | | Incubation Time: | 24 h | | Result: | Induced sub G1 events, and DNA fragmentation, which was correlated with the number of the apoptotic cells. |
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体内研究
(In Vivo) | Pectic acid (25 and 100 mg/kg, oral gavage) increases colon length, downregulates disease activity index, histopathological score and proinflammatory cytokine levels in Ulcerative colitis (UC) mice[3].
Pectic acid (6.25 and 12.5 mg/kg, intravenous injection) rescues the reduction in colon length in UC mice[3].
| Animal Model: | Ulcerative colitis (UC) mice[3] | | Dosage: | 25, 100 mg/kg | | Administration: | Oral gavage | | Result: | Exhibited the longest colon, lowest DAI, and minimum histopathological score.
Decreased the proinflammatory cytokines in the colonic tissue of UC mice. |
| Animal Model: | Ulcerative colitis (UC) mice[3] | | Dosage: | 6.25, 12.5 mg/kg | | Administration: | Intravenous injection | | Result: | Decreased the spleen and thymus index.
Restored the Th17/Treg balance in the spleen and lamina propria of UC mice.
Improved the gut microbiota composition.
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| CAS 号 | |
| 结构分类 | - Saccharides
- Polysaccharides
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| 来源 | - Plants
- Rutaceae
- Citrus reticulataBlanco
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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