3-Methyladenine (3-MA) 是PI3K的抑制剂。它通过抑制class III PI3K广泛作为自噬 (autophagy) 的抑制剂使用。
| 生物活性 | 3-Methyladenine (3-MA) is aPI3Kinhibitor. 3-Methyladenine is a widely used inhibitor ofautophagyvia its inhibitory effect on class IIIPI3K[1]. |
| IC50& Target[1] | PtdIns3Kγ 60 μM (IC50, Cell Assay) | Vps34 25 μM (IC50, Cell Assay) | Autophagy | Mitophagy | Human Endogenous Metabolite |
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体外研究
(In Vitro) | 3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:在体外细胞培养实验中，3-MA 常用的浓度为 0.5-10 mM，所以优先推荐直接称取本次实验所需粉末量，用培养基完全溶解，0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用，尽快用完。
Cell Viability Assay[2] | Cell Line: | HeLa cells | | Concentration: | 0 mM, 2.5 mM or 5 mM, 10 mM | | Incubation Time: | 0 hour, 24 hours and 48 hours | | Result: | Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation. |
Cell Autophagy Assay[2] | Cell Line: | HeLa cells | | Concentration: | 5 mM | | Incubation Time: | 24 hours | | Result: | Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions. |
Western Blot Analysis[2] | Cell Line: | HeLa cells | | Concentration: | 5 mM | | Incubation Time: | 0 hour, 12 hours, 24 hours and 48 hours | | Result: | Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours. |
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体内研究
(In Vivo) | 3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
| Animal Model: | 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3] | | Dosage: | 1.5 mg/100 g (1000 μM) | | Administration: | Intraperitoneal injection | | Result: | Alleviated Sodium Taurocholate-Induced SAP. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: H2O : 5 mg/mL(33.52 mM;Need ultrasonic) DMSO : 8.33 mg/mL(55.85 mM;Need ultrasonic) 配制储备液 | 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL | | 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL | | 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在本网站选购。 |
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