您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Forskolin
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Forskolin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Forskolin图片
CAS NO:66575-29-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议
1 g电议

产品名称
毛喉素
Coleonol
Colforsin
HL 362
产品介绍
Forskolin (Coleonol) 是一种有效的腺苷酸环化酶 (adenylate cyclase) 激活剂,对于 I 型腺苷酸环化酶,IC50为 41 nM,EC50为 0.5 μM。Forskolin 也是一种细胞内cAMP形成的诱导剂。Forskolin 诱导多种细胞类型的分化并激活孕烷 X 受体 (PXR) 和FXR。Forskolin 对心脏产生正性肌力作用,并具有血小板抗凝集和降压作用。Forskolin 也可诱导细胞自噬 (autophagy)。
生物活性

Forskolin (Coleonol) is a potentadenylate cyclaseactivator with anIC50of 41 nM and anEC50of 0.5 μM fortype I adenylyl cyclase[1]. Forskolin is also an inducer of intracellularcAMPformation[2]. Forskolin induces differentiation of various cell types and activatespregnane X receptor (PXR)andFXR[3]. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also inducesautophagy[4][5].

IC50& Target

IC50: 41 nM (Adenylyl cyclase)[1]
EC50: 0.5 μM (Adenylyl cyclase)[1]

体外研究
(In Vitro)

Forskolin (Coleonol) is also a potent exosome biogenesis and/or secretion activator in prostate cancer (PC) cells[8].
Forskolin (Fsk) is a naturally occurring diterpene isolated from Coleusforskholii, directly activates adenylyl cyclase (AC) through its catalytic subunit to increase intracellular levels of cyclic adenosine monophosphate (cAMP)[1].
Forskolin (Fsk) affects the proliferation of the human T-cell lines such as Kit 225 and MT-2. Forskolin treatment inhibits the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC50equal to ~5 μM Fsk. Forskolin treatment (10-100 μM) increases cAMPi levels ~5- to 20-fold above basal levels, which reache maximum levels between 50-100 μM Forskolin[6].

体内研究
(In Vivo)

The Forskolin (Coleonol)-treated Mrp4-/-mice shows an increased number of Ki67-positive and cleaved caspase 3-positive ECs, a significant decrease in the amount of pericyte coverage, and a reduced number of empty sleeves. In pups exposed to hyperoxia (75% oxygen) from P7 to P12, the Mrp4-/-mice shows a significant increase in the unvascularized retinal area[2].
The average blood glucose in the healthy rat group is 102.12±1.94 mg/dL, 101.25±3.56 for control group and 103±2.08 in forskolin group. The data shows that glucose levels at the end of the study are lower in forskolin group, with a significant difference according to the statistical tests applied (p=0.03)[7].

分子量

410.50

性状

Solid

Formula

C22H34O7

CAS 号

66575-29-9

中文名称

毛喉素

结构分类
  • Terpenoids
  • Diterpenoids
来源
  • Plants
  • Compositae
  • Gochnatia polymorpha(Less) Cabr.
  • Plants
  • Labiatae
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(243.61 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.4361 mL12.1803 mL24.3605 mL
5 mM0.4872 mL2.4361 mL4.8721 mL
10 mM0.2436 mL1.2180 mL2.4361 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.09 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.09 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。