Quercetin, a natural flavonoid, is a stimulator of recombinantSIRT1and also aPI3Kinhibitor withIC₅₀of 2.4 μM, 3.0 μM and 5.4 μM forPI3Kγ,PI3Kδ andPI3Kβ, respectively^[1].

Quercetin is a type of plant-based chemical, or phytochemical, used as an ingredient in supplements, beverages or foods. In several studies, it may have anti-inflammatory and antioxidant properties, and it is being investigated for a wide range of potential health benefits. Quercetin is a PI3K inhibitor with IC₅₀of 2.4-5.4 μM. Quercetin strongly abrogates PI3K and Src kinases, mildly inhibits Akt1/2, and slightly affected PKC, p38 and ERK1/2^[1]. Quercetin inhibits TNF-induced LDH% release, EC-dependent neutrophils adhesion to bovine pulmonary artery endothelial cells (BPAEC), and BPAEC DNA synthesis and proliferation^[2].

Combination of Quercetin (75 mg/kg) and 2-Methoxyestradiol enhances inhibition of human prostate cancer LNCaP and PC-3 cells xenograft tumor growth^[3].

302.24

Solid

C₁₅H₁₀O₇

117-39-5

槲皮素；栎精；槲皮黄素

• Flavonoids
• Flavonols

• Plants
• other families

Room temperature in continental US; may vary elsewhere.

DMSO : ≥ 100 mg/mL(330.86 mM)

Ethanol : 16.67 mg/mL(55.15 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 0.5%CMC-Na/saline water

  Solubility: 25 mg/mL (82.72 mM); Suspended solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 45%PEG300   5%Tween-80   50% saline

  Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic
• 3.

  请依序添加每种溶剂： 50% PG  50% Saline

  Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic
• 4.

  请依序添加每种溶剂： 50%PG  50%Saline

  Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic
• 5.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 6.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明