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DAPI Dihydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DAPI Dihydrochloride图片
CAS NO:28718-90-3
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品名称
4',6-Diamidino-2-phenylindole dihydrochloride
产品介绍
DAPI dihydrochloride 是结合富含 A-T 碱基对 DNA 序列的荧光染色。
生物活性

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

体外研究
(In Vitro)

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].

分子量

350.25

性状

Solid

Formula

C16H17Cl2N5

CAS 号

28718-90-3

Emission (Em)
Em 450-495 nm Blue
Excitation (Ex)
Ex 450-495 nm Blue
中文名称

4′,6-二脒基-2-苯基吲哚

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 75 mg/mL(214.13 mM;Need ultrasonic)

H2O : 5.56 mg/mL(15.87 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.8551 mL14.2755 mL28.5510 mL
5 mM0.5710 mL2.8551 mL5.7102 mL
10 mM0.2855 mL1.4276 mL2.8551 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
    Method: For cell staining.
    1. Cells are cultured in Dulbecco’s modified Eagle medium (DMEM), and supplemented with 10% fetal bovine serum.
    2. Cells are expanded in an incubator for 12 h at 37℃ with 5% CO2and then seeded onto confocal dishes.
    3. Cells are grown until confluence and washed with Hank’s balanced salt solution (HBSS).
    4. Cells are stained with DAPI dihydrochloride and imaged by a confocal laser scanning microscopy.
  • Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
    Method: For cell staining (TUNEL assay).
    1. Mice are sacrificed, and their small intestine tissues are harvested and fixed with 4% formaldehyde.
    2. The tissue sections are incubated with a TUNEL reaction mixture (1 h; 37℃; dark).
    3. The sections are stained with DAPI dihydrochloride (0.1 μg/mL).
    4. Cells are observed under an inverted fluorescence microscope (Olympus, Japan).
  • Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
    Method: For cell staining.
    1. Incubate cultured cells in DAPI dihydrochloride (1 μg/mL; 5 min).
    2. Observe staining cells under a fluorescence microscope.
  • Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.
    Method: For cell staining.
    1. Wash cultured cells for three times with PBS, cells are mounted with medium containing DAPI dihydrochloride.
    2. Samples are imaged with an Axioskop 2 mot plus fluorescence microscope equipped with Plan Apochromat 203/0.8 NA and 403/0.95 NA objectives and an AxioCam MRc camera with AxioVision software 4.7.1 (Carl Zeiss AG, Jena, Germany).