DAPI dihydrochloride 是结合富含 A-T 碱基对 DNA 序列的荧光染色。
生物活性 | DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA. |
体外研究 (In Vitro) | DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].
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Emission (Em) | Em 450-495 nm Blue |
Excitation (Ex) | Ex 450-495 nm Blue |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | -20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
溶解性数据 | In Vitro: DMSO : 75 mg/mL(214.13 mM;Need ultrasonic) H2O : 5.56 mg/mL(15.87 mM;ultrasonic and warming and heat to 60℃) 配制储备液 1 mM | 2.8551 mL | 14.2755 mL | 28.5510 mL | 5 mM | 0.5710 mL | 2.8551 mL | 5.7102 mL | 10 mM | 0.2855 mL | 1.4276 mL | 2.8551 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 3. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.94 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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染色示例 | Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.Method: For cell staining.1. Cells are cultured in Dulbecco’s modified Eagle medium (DMEM), and supplemented with 10% fetal bovine serum.2. Cells are expanded in an incubator for 12 h at 37℃ with 5% CO2and then seeded onto confocal dishes.3. Cells are grown until confluence and washed with Hank’s balanced salt solution (HBSS).4. Cells are stained with DAPI dihydrochloride and imaged by a confocal laser scanning microscopy. Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.Method: For cell staining (TUNEL assay).1. Mice are sacrificed, and their small intestine tissues are harvested and fixed with 4% formaldehyde.2. The tissue sections are incubated with a TUNEL reaction mixture (1 h; 37℃; dark).3. The sections are stained with DAPI dihydrochloride (0.1 μg/mL).4. Cells are observed under an inverted fluorescence microscope (Olympus, Japan). Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.Method: For cell staining.1. Incubate cultured cells in DAPI dihydrochloride (1 μg/mL; 5 min).2. Observe staining cells under a fluorescence microscope. Description: DAPI dihydrochloride is a DNA-specific probe with blue fluorescence.Method: For cell staining.1. Wash cultured cells for three times with PBS, cells are mounted with medium containing DAPI dihydrochloride.2. Samples are imaged with an Axioskop 2 mot plus fluorescence microscope equipped with Plan Apochromat 203/0.8 NA and 403/0.95 NA objectives and an AxioCam MRc camera with AxioVision software 4.7.1 (Carl Zeiss AG, Jena, Germany).
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