CAS NO: | 65-61-2 |
包装 | 价格(元) |
10 mM * 1 mL in DMSO | 电议 |
100mg | 电议 |
200mg | 电议 |
500mg | 电议 |
生物活性 | Acridine Orange hydrochloride is a cell-permeablefluorescent dyethat binds to nucleic acids, resulting in an altered spectral emission. | ||||||||||||||||
体外研究 (In Vitro) | Acridine Orange has been employed extensively as a cytochemical stain and has shown to stain differentially DNA and RNA, and double-restranded nucleic acidsin situ. Acridine Orange can either intercalate into double helical nucleic acids (green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (red fluorescence at 640 nm). This unique characteristic makes acridine orange useful for cell-cycle studies[1]. Acridine Orange staining of unfixed cells may be used as a simple, fast means of obtaining information on cell ploidy levels and cell cycle status from DNA measurements (green fluorescence), and cell transcriptional activity from RNA staining (red fluorescence), in human and murine cells lines, peripheral blood and bone marrow specimens from patients with leukemia and mitogenically (phytohemagglutinin) or antigenically (mixed lymphocyte culture) stimulated human peripheral blood cultures[2]. | ||||||||||||||||
分子量 | 301.81 | ||||||||||||||||
性状 | Solid | ||||||||||||||||
Formula | C17H20ClN3 | ||||||||||||||||
CAS 号 | 65-61-2 | ||||||||||||||||
Emission (Em) | Em 495-570 nm Green | ||||||||||||||||
Excitation (Ex) | Ex 495-570 nm Green | ||||||||||||||||
中文名称 | 吖啶橙;碱性橙 14;丫啶橙;3,6-双(二甲氨基)吖啶 | ||||||||||||||||
运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
储存方式 | 4°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) | ||||||||||||||||
溶解性数据 | In Vitro: H2O : 50 mg/mL(165.67 mM;ultrasonic and heat to 60℃) DMSO : 25 mg/mL(82.83 mM;Need ultrasonic) 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百
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