Hoechst 33258 analog

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CAS NO:

258843-62-8

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
50mg	电议
100mg	电议
200mg	电议
500mg	电议

产品介绍

Hoechst 33258 analog 是 Hoechst 染料的类似物。Hoechst 33258 analog 是一种细胞染料，可用于量化 DNA。Hoechst 33258 analog 与 dsDNA 结合时发出蓝色荧光。

生物活性

Hoechst 33258 analog is an analogue of the Hoechst dye. Hoechst 33258 analog is a cellular dye that can be used to quantify DNA. Hoechst 33258 analog emits blue fluorescence when bound to dsDNA^[1].

体外研究
(In Vitro)

Hoechst 33258 analog is used to detect the presence of nucleic acids in cells. Hoechst 33258 analog specifically to the target DNA sequence and emits blue fluorescence when bound to dsDNA. Hoechst 33258 analog is conjugated to a reporter probe and a signal generator^[1].

体内研究
(In Vivo)

Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells^[1].

分子量

510.59

性状

Solid

Formula

C₂₉H₃₀N₆O₃

CAS 号

258843-62-8

Emission (Em)

Em 450-495 nm Blue

Excitation (Ex)

Ex 450-495 nm Blue

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : ≥ 51 mg/mL(99.88 mM)

*"≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.9585 mL	9.7926 mL	19.5852 mL
5 mM	0.3917 mL	1.9585 mL	3.9170 mL
10 mM	0.1959 mL	0.9793 mL	1.9585 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

染色示例

Description: Hoechst 33258 analog can be used to stain DNA for detecting apoptosis.
Method: For cell staining.
1. 48 h after transfection, cell slides are prepared and fixed in 4% paraformaldehyde.
2. Stain cells with Hoechst 33258 analog (30 min; dark) solution.
3. Use a fluorescence microscope (Nikon Instruments Inc., Tokyo, Japan) for image.