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Methylene Blue
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Methylene Blue图片
CAS NO:61-73-4
包装与价格:
包装价格(元)
100mg电议
500mg电议
1 g电议
5 g电议

产品名称
亚甲蓝
Basic Blue 9
CI-52015
METHYLTHIONINIUM CHLORIDE
产品介绍
Methylene blue (Basic Blue 9) 是一种鸟苷酸环化酶 (sGC),单胺氧化酶 A (MAO-A) 和 NO 合酶 (NOS) 抑制剂。Methylene blue 是一种血管加压剂,在医疗中通常用作染料。Methylene blue 通过一氧化氮合成酶/鸟苷酸环化酶信号通路降低脉冲前抑制。Methylene blue 是一种氧化还原循环化合物,能够穿过血脑屏障。Methylene blue 是 Tau 聚集抑制剂。亚甲蓝可减轻脑水肿,减弱小胶质细胞活化,减少神经炎症。
生物活性

Methylene blue (Basic Blue 9) is aguanylyl cyclase (sGC),monoamine oxidaseA (MAO-A)andNO synthase(NOS)inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

IC50& Target

Guanylyl cyclase (sGC)[1].
Monoamine oxidase A (MAO-A)[1].
NO synthase (NOS)[1]

体外研究
(In Vitro)

Methylene blue (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

体内研究
(In Vivo)

Methylene blue (Basic Blue 9) (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
Methylene blue (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

Animal Model:Male NMRI mice[1]
Dosage:50 and 100 mg/kg
Administration:Intraperitoneal injection; once, for 25 minutes
Result:Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
Animal Model:CaMKIIα-tTA transactivator mice[2]
Dosage:20 and 40 mg/kg
Administration:Oral administration; daily, for 6 months
Result:Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
Animal Model:TBI-treated male BALB/c mice[3]
Dosage:2 mg/kg
Administration:Intravenous injection; once, for 1 day
Result:Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
Animal Model:TBI-treated male BALB/c mice[3]
Dosage:2 mg/kg
Administration:Intravenous injection; once, for 1 day
Result:Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Clinical Trial
分子量

319.85

性状

Solid

Formula

C16H18ClN3S

CAS 号

61-73-4

中文名称

亚甲蓝

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 50 mg/mL(156.32 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.1265 mL15.6323 mL31.2647 mL
5 mM0.6253 mL3.1265 mL6.2529 mL
10 mM0.3126 mL1.5632 mL3.1265 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

染色示例
  • Description: Methylene Blue can be used for cellular staining in colony formation assay.
    Method: For cell staining.
    1. Fix colonies with methanol/acetone (1:1).
    2. Stain cells with 0.5% Methylene Blue.
  • Description: Methylene Blue can be used for cellular staining in colony formation assay.
    Method: For cell staining.
    1. Fix colonies with methanol/acetone (1:1).
    2. Stain cells with 0.5% Methylene Blue.
    3. Use a Nikon TE200 microscope for colonies counting.