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Biacetyl monoxime
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Biacetyl monoxime图片
CAS NO:57-71-6
包装与价格:
包装价格(元)
100mg电议
200mg电议
500mg电议

产品名称
Diacetyl monoxime
DAM
产品介绍
Biacetyl monoxime (Diacetyl monoxime) 是一种肌球蛋白 ATP 酶 (myosin ATPase) 抑制剂,是一种骨骼肌和心肌收缩抑制剂。Biacetyl monoxime 也是一种特征良好的骨骼肌肌球蛋白 II(myosin-II)的化学和运动活性的非竞争性抑制剂。Biacetyl monoxime 诱导肌质网 Ca2+释放。
生物活性

Biacetyl monoxime (Diacetyl monoxime), amyosinATPaseinhibitor, is a skeletal and cardiac muscle contraction inhibitor. Biacetyl monoxime is also a well-characterized non-competitive inhibitor of chemical and motile activity of skeletal musclemyosin-II. Biacetyl monoxime induces sarcoplasmic reticulum Ca2+release[1][2][3].

体外研究
(In Vitro)

Biacetyl monoxime (Diacetyl monoxime) (50 mM, 6 and 48 h) decreases cellulase secretion inC. cinerea[1].
Biacetyl monoxime (50 mM, 2 and 4 h) disrupts the localization of the Golgi apparatus, but not that of the endoplasmic reticulum[1].
Biacetyl monoxime (0-30 mM) induces SR Ca2+release (no efflux inhibitors) in a concentration-dependent manner, with a maximal reduction of 72% of SR Ca2+at pCa 6.0[2].
Biacetyl monoxime acts as a chemical phosphatase, which has led to speculation that dephosphorylation of key Ca2+channel proteins may be involved in its inhibition of contraction[2].
Biacetyl monoxime does not inhibit the ATPase activity of two different myosin-I isoforms, myosin-V, or myosin-VI[3].
Biacetyl monoxime (0-50 mM) suppresses L-type Ca2+current of single cardiac myocytes isolated from SHR and WKY rats[4].
Biacetyl monoxime significantly reduces the duration of both spontaneous and electrically stimulated action potentials of cultured neonatal rat cardiomyocytes[4].

体内研究
(In Vivo)

Biacetyl monoxime (0-200 mg/kg; i.v.; once) shows hypotensive effect[4].
Biacetyl monoxime (0-205 mg/kg; i.p.; once) shows anticonvulsant effect against Picrotoxin (HY-101391)-induced convulsions[5].

Animal Model:Male SHR and age-matched WKY rat[4]
Dosage:5, 30, I00 and 200 mg/kg
Administration:Intravenous administration, 1 mL/kg, once
Result:Decreased arterial blood pressure for both strains, the SHR was significantly more responsive.
Animal Model:Male mice (20 to 25 g)[5]
Dosage:51, 103 and 205 mg/kg in combination with intraperitoneal injection of 3.0 mg/kg Picrotoxin (HY-101391)
Administration:Intraperitoneal injection, once
Result:Showed dose-dependent anticonvulsant effect against Picrotoxin-induced convulsions.
分子量

101.10

性状

Solid

Formula

C4H7NO2

CAS 号

57-71-6

中文名称

二乙酰一肟

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 125 mg/mL(1236.40 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM9.8912 mL49.4560 mL98.9120 mL
5 mM1.9782 mL9.8912 mL19.7824 mL
10 mM0.9891 mL4.9456 mL9.8912 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (20.57 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (20.57 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (20.57 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。