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Gardenin A
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Gardenin A图片
CAS NO:21187-73-5
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
栀子(黄)素A
产品介绍
Gardenin A 是一种口服有效的、合成的 PMF 类似物,对神经突生长和神经元分化具有神经营养作用。Gardenin A 通过激活 PC12 细胞中的MAPK/ERKPKCPKA信号通路促进神经细胞的发生,但不激活 TrkA、CREB 信号通路。Gardenin A 也有镇静、抗焦虑、抗抑郁和抗惊厥作用。
生物活性

Gardenin A is an orally active and synthetic PMF analogue with the neurotrophic effect for neurite outgrowth and neuronal differentiation. Gardenin A promotes neuritogenesis via activatingMAPK/ERK,PKC, andPKA, but notTrkA, CREB signaling pathways. Gardenin A also has sedative, anxiolytic, antidepressant, and anticonvulsant effects[1][2].

体外研究
(In Vitro)

Gardenin A (10-20 μM; 48 h) 可诱导 PC12 细胞神经突生长,(10 μM; 24 小时和 48 小时) 还增加神经元分化和突触形成标记蛋白、生长相关蛋白-43 (GAP-43) 和突触素的表达[1]
Gardenin A (10 μM; 30-120分钟) 显著诱导环 AMP 反应元件结合蛋白(CREB) 的磷酸化和 CRE 介导的转录,这是通过抑制剂 2-萘酚AS-E 磷酸盐 (KG-501) 或使用 CREB siRNA 来抑制的[1]
Gardenin A (10 μM; 15-120分钟) 增加 ERK 磷酸化水平,提高 PKA 和 PKC 活性[1]

Cell Viability Assay[1]

Cell Line:PC12 cells
Concentration:2, 5, 10, 20 μM
Incubation Time:48 hours
Result:showed cell growth supporting effect at higher concentrations (10-20 μM) and did not exert detectable 237 cytotoxicity on PC12 cells after 48 h incubation in low serum medium.

Western Blot Analysis[1]

Cell Line:PC12 cells
Concentration:10 μM
Incubation Time:0, 15, 30, 60, and 120 min
Result:Significantly increased the phosphorylation of ERK1/2 (Thr202/Tyr204) at 15 min, subsequently reduced after 30 min.
Increased PKC and PKA activity peaked at 15 min. Increased CREB phosphorylation.
体内研究
(In Vivo)

Gardenin A (0.1-25 mg/kg; 口服; 单剂量) 具有神经药理作用,包括镇静、抗焦虑、抗抑郁和抗惊厥作用的小鼠[2]
栀子苷A (1-10 mg/kg; 口服; 单次剂量) 不影响小鼠的运动协调和延迟惊厥的发生[2]

Animal Model:Male Balb/c mice (24-32 g)[2]
Dosage:1 mg/kg, 10 mg/kg, 25 mg/kg
Administration:Oral gavage; single dose
Result:Showed sedative effects at a dose of 25 mg/kg, which increased the duration of sleep but did not alter sleep onset.
分子量

418.39

Formula

C21H22O9

CAS 号

21187-73-5

中文名称

栀子(黄)素A

结构分类
  • Flavonoids
  • Flavones
来源
  • Plants
  • Rutaceae
  • Citrus reticulataBlanco
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.