Amylin,amide,human

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Amylin,amide,human

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

122384-88-7

包装与价格：

包装	价格(元)
500 μg	电议
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品名称

DAP amide, human

产品介绍

Amylin, amide, human 是一种 37 个氨基酸的多肽，是一种与胰岛素共分泌的胰腺激素，在代谢和葡萄糖稳态中发挥独特作用。Amylin, amide, human 抑制胰高血糖素分泌，延迟胃排空，并作为饱腹感剂。

生物活性

Amylin, amide, human, a 37-amino acid polypeptide, is a pancreatic hormone cosecreted withinsulinthat exerts unique roles in metabolism and glucose homeostasis. Amylin, amide, human inhibits glucagon secretion, delays gastric emptying, and acts as a satiety agent^[1].

体外研究
(In Vitro)

MCF-7 cells endogenously express human amylin receptor CTR1 and CTR2. Stimulation of the receptor with Amylin, amide, human results in the production of cAMP. Amylin, amide, human (0.001 nM-1000 μM) results in an EC₅₀of 35.2±7.5 nM^[1].

Cell Viability Assay^[1]

Cell Line:	MCF-7 cells
Concentration:	0.001 nM, 0.01 nM, 0.1 nM, 1 nM, 10 nM, 100 nM, 1 μM, 10μM, 100 μM, 1000 μM
Incubation Time:
Result:	Resulted in the production of cAMP with an EC₅₀of 35.2±7.5 nM.

体内研究
(In Vivo)

Amylin, amide, human (400 μg peptide /kg body weight) is injected by subcutaneous route in separated groups of swiss male mice. A typical PK curve for the free amylin is observed, with a half-time of 23 min^[1].

Animal Model:	Swiss male mice (8 weeks old)^[1]
Dosage:	400 μg peptide /kg body weight (Pharmacokinetic Analysis)
Administration:	Subcutaneous route; 24 hours
Result:	Half-time of 23 min.

分子量

3903.28

性状

Solid

Formula

C₁₆₅H₂₆₁N₅₁O₅₅S₂

CAS 号

122384-88-7

Sequence

Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-His-Ser-Ser-Asn-Asn-Phe-Gly-Ala-Ile-Leu-Ser-Ser-Thr-Asn-Val-Gly-Ser-Asn-Thr-Tyr-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY-NH2 (Disulfide bridge: Cys2-Cys7)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.