Decursin

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Decursin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

5928-25-6

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议

产品名称

紫花前胡素
(+)-Decursin

产品介绍

Decursin ((+)-Decursin) 是一种有效的抗肿瘤剂。Decursin 还是一种细胞毒剂和有效的蛋白激酶 C (protein kinase C) 激活剂。Decursin 诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G1 期。Decursin 在 48 小时降低 CDK2、CDK4、CDK6、cyclin D1 蛋白的表达。Decursin 抑制细胞增殖和迁移。Decursin 具有抗肿瘤、抗炎和镇痛活性。

生物活性

Decursin ((+)-Decursin) is a potentanti-tumoragent. Decursin also is a cytotoxic agent and a potentprotein kinase Cactivator. Decursin inducesapoptosisand cell cycle arrest at G1 phase. Decursin decreases the expression ofCDK2,CDK4,CDK6, cyclin D1 protein at 48 h. Decursin inhibits cell proliferation and migration. Decursin shows anti-tumor, anti-inflammatory and analgesic activities^[1]^[2]^[3]^[4].

体外研究
(In Vitro)

Decursin (0, 25, 50, 100 μM; 24, 28, 72, 96 h) inhibits cell growth in a dose- and time- dependent manner in DU145 cells^[1].
Decursin (0, 25, 50, 100 μM; 24, 28, 72, 96 h) induces apoptosis and cell cycle arrest at G1 phase in DU145 cells, G1, S as well as G2-M arrests in PC-3 cells^[1].
Decursin (0, 25, 50, 100 μM; 24, 48 h) decreases the expression of CDK2, CDK4, CDK6, cyclin D1 protein at 48 h in DU145 cells^[1].
Decursin (0, 5, 20, 100 μM; 7 days) inhibits the proliferation and differentiation ability of AC133+ cells^[2].
Decursin (0, 5, 20, 100 μM) inhibits SDF-1a-induced activation of Akt, ERK1/2, and eNOS in a dose-dependent manner^[2].

Cell Viability Assay^[1]

Cell Line:	DU145 cells
Concentration:	0, 25, 50, 100 μM
Incubation Time:	24, 28, 72, 96 h
Result:	Showed a dose- and time- dependent inhibition cell growth with 22% to 51%, 21%to 68%, 9% to 72%, 42% to 90% growth inhibition after 24, 48, 72, and 96 hours oftreatment, respectively. And caused 15%-45% cell death.

Cell Cycle Analysis^[1]

Cell Line:	DU145 cells
Concentration:	0, 25, 50, 100 μM
Incubation Time:	12-96 h
Result:	Caused 52%, 65%, and 78% DU145 cells in G1phase.

Western Blot Analysis^[1]

Cell Line:	DU145 cells
Concentration:	0, 25, 50, 100 μM
Incubation Time:	24, 48 h
Result:	Did not show any any alteration inprotein levels of CDK2, CDK4, CDK6, cyclin D1, and cyclin E, but dose-dependent decreased in the expression of these proteins except cyclin E at 48 h.

体内研究
(In Vivo)

Decursin (4 mg/kg; s.c.; daily for 4 weeks) shows anti-tumor activity in mouse^[2].
Decursin (50 mg/kg; intrathecal injection; three times at 2-day intervals, for 6 days) shows analgesic ability in paclitaxel-induced peripheral neuropathy in mouse^[3].

Animal Model:	C57BL/6J mice (LLC cells)^[2]
Dosage:	4 mg/kg
Administration:	S.c.; daily for 4 weeks
Result:	Delayed tumor formation and dramatically decreased tumor growth by inhibition of angiogenesis through VEGFR-2 signaling pathway.

Animal Model:	Eight-week-old adult C57BL/6J male and female mice^[3]
Dosage:	50 mg/kg
Administration:	Intrathecal injection; three times at 2-day intervals, for 6 days
Result:	Demonstrated the analgesic ability in the in vivo model of paclitaxel-induced peripheral neuropathy.

分子量

328.36

性状

Solid

Formula

C₁₉H₂₀O₅

CAS 号

5928-25-6

中文名称

紫花前胡素

结构分类

Phenylpropanoids
Coumarins

来源

Plants
Umbelliferae
Angelica sinensis(Oliv.) Diels

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 50 mg/mL(152.27 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.0454 mL	15.2272 mL	30.4544 mL
5 mM	0.6091 mL	3.0454 mL	6.0909 mL
10 mM	0.3045 mL	1.5227 mL	3.0454 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
2.
请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline)
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.
请依序添加每种溶剂： 10% DMSO 90%corn oil
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在本网站选购。