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Taurodeoxycholic acid sodium hydrate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Taurodeoxycholic acid sodium hydrate图片
CAS NO:110026-03-4
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
50mg电议
100mg电议
200mg电议

产品名称
Sodium taurodeoxycholate monohydrate
产品介绍
Taurodeoxycholic acid sodium hydrate (Sodium taurodeoxycholate monohydrate) 是一种胆汁酸,是由肝脏中的胆固醇合成的两亲性表面活性剂分子。 除TGR5通路外,Taurodeoxycholic acid sodium hydrate 还激活S1PR2通路。
生物活性

Taurodeoxycholic acid sodium hydrate (Sodium taurodeoxycholate monohydrate), a bile acid, is an amphiphilic surfactant molecule synthesized fromcholesterolin the liver. Taurodeoxycholic acid sodium hydrate activates theS1PR2pathway in addition to theTGR5pathway[1].

IC50& Target[1]

Microbial Metabolite

 

体外研究
(In Vitro)

The median plasma concentration of Taurodeoxycholate is 33.9 nM in healthy individuals[1].
Taurodeoxycholate inhibits the binding of N-3H-methylscopolamine to the M3 muscarinic receptor of acetylcholine with an IC50of 170 μM[1].
Taurodeoxycholate (0.05-1.00 mM; 1-6 days) stimulates intestinal epithelial cell proliferation[2].
Taurodeoxycholate (0.05-1.00 mM; 24 h) induces a significant increase in S-phase concentration and a significant decrease in G1-phase concentration of the cell cycle, increases c-myc protein and mRNA expression in IEC-6 cells[2].

Cell Proliferation Assay[2]

Cell Line:IEC-6 and caco-2 cells
Concentration:0, 0.05, 0.50, and 1.00 mM
Incubation Time:1, 2, 4 and 6 days
Result:Significantly stimulated intestinal epithelial cell proliferation in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line:IEC-6 cells
Concentration:0, 0.05, 0.50, and 1.00 mM
Incubation Time:24 h
Result:Significantly increased cells in S phase and decreased cells in G1-phase.

Western Blot Analysis[2]

Cell Line:IEC-6 cells
Concentration:0.5 mM
Incubation Time:1 and 6 days
Result:Significantly increased c-myc protein expression.
体内研究
(In Vivo)

Taurodeoxycholate (0.5 mg/kg; i.v.; once) confers protection to C57BL/6N mice with sepsis, but does not protect TGR5 KO mice under sepsis[1].

Animal Model:C57BL/6N mice,Lipopolysaccharides(HY-D1056) injection model of sepsis[1]
Dosage:0.5 mg/kg
Administration:Intravenous injection, 30 min or 24 h after LPS injection
Result:Improved the survival rate of mice with sepsis. Decreased liver and kidney damage in septic mice. Ameliorated systemic inflammation and normalized blood pressure in septic mice.
分子量

539.70

性状

Solid

Formula

C26H46NNaO7S

CAS 号

110026-03-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 125 mg/mL(231.61 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.8529 mL9.2644 mL18.5288 mL
5 mM0.3706 mL1.8529 mL3.7058 mL
10 mM0.1853 mL0.9264 mL1.8529 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 0.5%CMC-Na/saline water

    Solubility: 20 mg/mL (37.06 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.85 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.85 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.85 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。