Colivelin TFA

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Colivelin TFA

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
500 μg	电议
1mg	电议
5mg	电议
10mg	电议
50mg	电议
100mg	电议

产品介绍

Colivelin TFA 是一种具有大脑通透性的神经保护肽 (neuroprotective peptide)，是STAT3的有效激活剂。Coliveli 通过激活 STAT3 在体外抑制神经元死亡。Colivelin TFA 对神经毒性、Aβ 沉积,神经元细胞凋亡，在神经退行性疾病显示出长期有利的影响。Colivelin TFA 可用于阿尔茨海默病和缺血性脑损伤的相关研究。

生物活性

Colivelin TFA is a brain penetrantneuroprotective peptideand a potent activator ofSTAT3, suppresses neuronal death by activating STAT3 in vitro^[1]. Colivelin TFA exhibits long-term beneficial effects against neurotoxicity, Aβ deposition, neuronalapoptosis, and synaptic plasticity deficits in neurodegenerative disease^[2]. Colivelin TFA has the potential for the treatment of alzheimer's disease and ischemic brain injury^[1].

IC₅₀& Target^[1]

STAT3

Amyloid-β

体外研究
(In Vitro)

Colivelin completely suppresses death induced by overexpressed FAD-causative genes and Aβ1-43 at a concentration of 100 fm, and keep its neuroprotective action at or above the levels of 1 nm^[1].
Colivelin-induced neuroprotection occurs via two neuroprotective pathways: one mediated by Ca²⁺/calmodulin-dependent protein kinase IV, triggered by ADNF, and one mediated by signal transducer and activator of transcription 3, triggered by HN^[1].
Colivelin reverses caspase3, Bax and Bcl-2 expressions in HT22 cells medaited by rmMFG-E8 in the co-cultured cells under OGD condition^[4].
Colivelin (50 μg/mL, 4 hours) significantly increases the p-STAT3 protein levels in BV-2 cells^[4].

Western Blot Analysis^[4]

Cell Line:	BV-2 cells.
Concentration:	50 μg/mL.
Incubation Time:	4 hours.
Result:	Increased p-STAT3 levels.

Cell Viability Assay^[5]

Cell Line:	KYSE70 and TE8 cells.
Concentration:	0.5 μM.
Incubation Time:	1 hour (followed by CYT-Rx20 treatment)
Result:	Significantly suppressed the viability in KYSE70 and TE8 cells.

体内研究
(In Vivo)

Colivelin(intracerebroventricular administration; 10 pmol/3 μl; 3 weeks) suppresses impairment in spatial working memory induced by repetitive intracerebroventricular injection of Aβ25-35 or Aβ1-42, in addition, it antagonizes neuronal loss in the CA1 region of hippocampus induced by hippocampal injection of Aβ1-42^[1].
Colivelin (intraperitoneal administration; 1.4, 7, or 35 nM/0.21mL; on the Y-maze testday) suppresses memory impairment caused by 3-quinuclidinyl benzilateand restricts functional memory deficit^[1].
Colivelin (intraperitoneal injection; 1 mg/kg; 14 days) results in improved motor and cognitive function with time by performance of mNSS, rotarod, and corner turning test.It also reduces lesion volume and improves neurological deficits after MCAO^[1].

Animal Model:	CD-1 mice^[1]
Dosage:	10 pmol/3 μl
Administration:	Intracerebroventricular administration
Result:	Completely suppressed Aβ 25-35-mediated impairment in spatial working memory and increased the number of immunoreactive neurons.

Animal Model:	C57 mice^[1]
Dosage:	1.4, 7, or 35 nM/0.21mL
Administration:	Intraperitoneal administration
Result:	Protected against cholinotoxin-induced amnesia in mice.

Animal Model:	Male C57BL/6 mice^[3]
Dosage:	1 mg/kg
Administration:	Intraperitoneal administration
Result:	Protected against ischemic brain injury, and improves neurological outcomes.

分子量

2759.12

性状

Solid

Formula

C_{1₁₉H₂₀6}N₃₂O₃₅.C₂HF₃O₂

Sequence Shortening

SALLRSIPAPAGASRLLLLTGEIDLP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(18.12 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.3624 mL	1.8122 mL	3.6243 mL
5 mM	0.0725 mL	0.3624 mL	0.7249 mL
10 mM	0.0362 mL	0.1812 mL	0.3624 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： Water with 5% sefsol and 20% isopropanol
Solubility: 6.25 mg/mL (2.27 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。