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EDC,HCl
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
EDC,HCl图片
CAS NO:25952-53-8
包装:10g
市场价:683元

产品介绍
EDC.HCl 是一种碳二亚胺试剂,可以形成核酸和具有酰胺键的化合物。
Cas No.25952-53-8
别名1-乙基-(3-二甲基氨基丙基)碳酰二亚胺盐酸盐
化学名3-(ethyliminomethylideneamino)-N,N-dimethylpropan-1-amine;hydrochloride
Canonical SMILESCCN=C=NCCCN(C)C.Cl
分子式C8H17N3.HCl
分子量191.7
溶解度≥ 19.2 mg/mL in DMSO, ≥ 39.6 mg/mL in ETOH, ≥ 39 mg/mL in Water
储存条件Store at -20°C, protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

IC50: Not available.

EDC.HCl, which is commonly regarded as carbodiimide reagents, serves as a very useful tool for the formation of amide bonds (peptide bonds) in an aqueous medium. As one of the carbodiimide reagents, it is also effectively applied in anhydroxydation, polynucleotide synthesis, esterification and lactonization.In addition, EDC.HCl is also widely used as water soluble condensing reagent. [1]

In vitro: Carbodiimide-mediated coupling method was one of the most traditional approaches for peptide-bond formation. With the aim to synthesis target compounds, carbodiimide reagents were added after thoroughly mixing of a carboxylic acid and an amine. After the reaction was accomplished, the carbodiimide reagent was then changed into its corresponding urea. [2] Recently, a simple and fast detection method using spectrophotometric flow injection analysis was developed. This method was based on the reaction between EDC.HCl and pyridine in acidic aqueous solution. After reaction at 40C for a while, the absorbance was measured at 400 nm. The calibration curve demonstrated a good linearity from 0 to 10% of EDC·HCl solutions with the following regression equation: y = 3.15 × 104x (x, % concentration of EDC·HCl; y, peak area). This approach served to monitoring the concentration of EDC·HCl after its reaction in water. [1]

In vivo: So far, no in vivo data has been reported.

Clinical trial: So far, no clinical trial has been conducted.

References:
[1]Kunihiko Seno, Kazuki Matumura, Mitsuko Oshima, and Shoji Motomizu.  Spectrophotometric determination of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr; 24: 505-8.
[2] Jad YE, Khattab SN, Beatriz G.  Torre DL, Govender T, Kruger HG, Faham AE and Albericio F. EDC·HCl and potassium salts of oxyma and oxyma-B as superior coupling cocktails for peptide synthesis. Eur J Org Chem. 2015 May; 2015(14): 3116-20.