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SRI-37240
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
SRI-37240图片
CAS NO:883956-47-6
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
SRI-37240是一种有效的提前终止密码子 (PTCs) 抑制剂。SRI-37240 抑制CFTR的无义突变。在 HEK293T 细胞中,SRI-37240 改变 PTCs 的细胞翻译终止。当与 G418 使用联合时,SRI-37240 还能恢复原发性支气管上皮细胞的 CFTR 功能。
生物活性

SRI-37240 is a potentpremature termination codons(PTCs) inhibitor. SRI-37240 suppressesCFTRnonsense mutations. SRI-37240 alters cellular translation termination at PTCs in HEK293T cells. SRI-37240 can also restoreCFTRfunction in primary bronchial epithelial cells when combination with G418[1].

IC50& Target

Premature termination codons, CFTR[1]

体外研究
(In Vitro)

SRI-37240 (1, 3, 10 and 30 μM; 48 h) induces concentration-dependent increases in CFTR-dependent (Forskolin-stimulated and sensitive to the inhibitor CFTRInh-172) chloride conductance[1].
SRI-37240 (10 μM; 72 h) significantly increases the amount of full-length, fully glycosylated form of CFTR protein, and the unprocessed, immature form of full-length CFTR protein in 16HBEge cells when co-treated with G418 (100 μM)[1].
SRI-37240 (10 μM; 24 h) alters cellular translation termination at PTCs in HEK293T cells, also increases global densities of ribosomes at normal stop codons without affecting densities of ribosomes in 3-UTRs[1].
SRI-37240 (10 μM; 72 h) restores CFTR function in primary bronchial epithelial cells when combination with G418[1].

Western Blot Analysis[1]

Cell Line:CFTR-G542X 16HBEge
Concentration:10 μM
Incubation Time:24 h
Result:Significantly increased the amount of Band C CFTR protein, which represents the full-length, fully glycosylated form of CFTR and Band B, which represents the unprocessed, immature form of full-length CFTR protein when combined with G418 (100 μM).
分子量

385.46

性状

Solid

Formula

C24H23N3O2

CAS 号

883956-47-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 11.36 mg/mL(29.47 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.5943 mL12.9715 mL25.9430 mL
5 mM0.5189 mL2.5943 mL5.1886 mL
10 mM0.2594 mL1.2972 mL2.5943 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。