GLUT1-IN-1 是一种葡萄糖转运蛋白 1(GLUT1)抑制剂，具有 GLUT1 特异性失活能力。GLUT1-IN-1 对 HeLa、A549 和 HepG2 细胞具有浓度依赖性细胞毒性，IC50值分别为 5.49 μM，11.14 μM 和 8.73 μM。GLUT1-IN-1 可用于光动力疗法（PDT）和癌症的研究。
| 生物活性 | GLUT1-IN-1 is aglucose transporter 1 (GLUT1)inhibitor and has a GLUT1-specific inactivation ability. GLUT1-IN-1 exhibits concentration-dependent cytotoxicity for HeLa, A549 and HepG2 cells withIC50values of 5.49 μM, 11.14 μM, and 8.73 μM, respectively. GLUT1-IN-1 can be used for the research of photodynamic therapy (PDT) and severalscancer[1]. |
| IC50& Target[1] | |
体外研究
(In Vitro) | GLUT1-IN-1(compound 8) (0-100 μM; 1 h) exhibits concentration-dependent cytotoxicity for HeLa, A549 and HepG2 cells with IC50values of 5.49, 11.14, and 8.73 μM, respectively (Under light irradiation)[1].
GLUT1-IN-1 (0-100 μM; 1 h) possesses sufficient photosensitizing ability to exhibit significant cytotoxicity and that the glucose conjugation contributes to the suppression of the cytotoxicity of I2BODIPY[1].
GLUT1-IN-1 (8 μM, 10 μM; 1 h) selectively interacts with GLUT1 and oxidizes it under light irradiation, resulting in the conversion of GLUT1 into a derivative that is undetectable by immunoblotting analysis[1].
GLUT1-IN-1(1 h) has a GLUT1-specific inactivation ability and causes lightinduced cytotoxicity by modulating the EGFR/MAPK signaling pathway[1].
Cell Viability Assay[1] | Cell Line: | HeLa, A549 and HepG2 cells | | Concentration: | 0-100 μM | | Incubation Time: | 1 h | | Result: | Exhibited a concentration-dependent cytotoxicity against these cancer cell lines under light irradiation. |
Western Blot Analysis[1] | Cell Line: | HeLa, A549 and HepG2 cells | | Concentration: | 10 μM | | Incubation Time: | 1 h | | Result: | Not detected GLUT1in cell lines treated with 10 uM under light irradiation.
Reduced the levels of epidermal growth factor receptor tyrosine kinase (EGFR), phospho-ERK (Y204), and GLUT1 without affecting ERK, atubulin, and PCNA protein levels. |
Immunofluorescence[1] | Cell Line: | HeLa, A549 and HepG2 cells | | Concentration: | 8, 10 μM | | Incubation Time: | 1 h | | Result: | Observed a reduction in the intensity of the fluorescent signals due to glucose transporter 1 (GLUT1). |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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