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Avicularin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Avicularin图片
CAS NO:572-30-5
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品名称
扁蓄苷;蓄苷
产品介绍
Avicularin 是一种具有口服活性的黄酮类化合物。Avicularin 抑制NF-κB (p65),COX-2PPAR-γ的活性。Avicularin 具有抗炎、抗感染、抗过敏、抗氧化、保肝、抗肿瘤等作用。
生物活性

Avicularin is an orally active flavonoid. Avicularin inhibitsNF-κB(p65),COX-2andPPAR-γactivities. Avicularin has anti-inflammatory, anti-infectious anti-allergic, anti-oxidant, hepatoprotective, and anti-tumor activities[1][3].

IC50& Target[2][3]

GLUT4

 

PPARγ

 

COX-2

 

体外研究
(In Vitro)

Avicularin (10-300 μM, 1 h) suppresses NO and PGE2production in LPS-stimulated RAW 264.7 cells[1].
Avicularin (10-300 μM, 1 h) exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 cells[1].
Avicularin (25-100 μg/mL, 48 h) decreases cell proliferation, cell migration and invasion in Huh7 cells[2].
Avicularin (25-100 μg/mL, 48 h) induces apoptosis via the downregulation of NF-κB (p65) and COX-2 and the upregulation of PPAR-γ[2].
Avicularin (50 μM, 6 days) decreases the intracellular lipids, along with decreased PPARγ, C/EBPα, and aP2 mRNA levels in 3T3-L1 cells[3].
Avicularin (50 μM, 6 days) suppresses GLUT4-Mediated glucose uptake in 3T3-L1 cells[3].
Avicularin (2.5-10 μM, 2 h) inhibits ECM degradation and inflammation via TRAF6/MAPK activation in rat and human chondrocytes[4].

Cell Proliferation Assay[2]

Cell Line:Huh7 cells
Concentration:25, 50, 100 μg/mL
Incubation Time:12, 24, 36 and 48 h
Result:Inhibited cell proliferation in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line:RAW 264.7 cells
Concentration:10, 30, 100, 300 μM
Incubation Time:1 h
Result:Inhibited LPS-induced protein expression of iNOS and COX-2, release of pro-inflammatory cytokine IL-1β, degradation of cytosolic IκB, and phosphorylation of ERK.

RT-PCR[3]

Cell Line:3T3-L1 cells
Concentration:50 μM
Incubation Time:6 days
Result:Decreased PPARγ, C/EBPα, and aP2 mRNA levels approximately 28.7, 69.5, and 18.3%, respectively.
体内研究
(In Vivo)

Avicularin (injected in articular cavity of the knee, 0.5-2 mg/kg, twice a week for 4 weeks) attenuates the development of OA (osteoarthritis) in ACLT-induced rats[4].
Avicularin (oral administration, 50 and 100 mg/kg, for 21 days) attenuates memory Impairment in rats with amyloid Beta-induced Alzheimer's disease[5].

Animal Model:ACLT (anterior cruciate ligament transection)-induced rats[4]
Dosage:0.5, 1, 2 mg/kg
Administration:Injected into the articular cavity of the right knee, twice a week for 4 weeks.
Result:Alleviated the tibial subchondral osteolysis, reduces bone loss, and increases the bone mass of tibial subchondral bone.
Attenuated ECM degradation, the loss of Aggrecan and Collagen II in ACLT-induced rats.
Decreased the MMP3 and MMP13 protein level.
Animal Model:Rats with amyloid Beta-induced Alzheimer's disease[5]
Dosage:25, 50, and 100 mg/kg
Administration:Oral administration, for 21 days
Result:Enhanced cognition activity, and reversed the effects of amyloid beta-induced inflammatory response and excessive oxidative stress.
分子量

434.35

性状

Solid

Formula

C20H18O11

CAS 号

572-30-5

中文名称

扁蓄苷;蓄苷

结构分类
  • Flavonoids
  • Flavonols
  • Phenols
  • Polyphenols
来源
  • Plants
  • Lauraceae
  • Lindera aggregata(Sims) Kosterm.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(230.23 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.3023 mL11.5115 mL23.0229 mL
5 mM0.4605 mL2.3023 mL4.6046 mL
10 mM0.2302 mL1.1511 mL2.3023 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.79 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.79 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在本网站选购。