Alda-1 is a potent and selectiveALDH2agonist, which activates wild-typeALDH2and restores near wild-type activity to ALDH2*2.

ALDH2^[1]

Alda-1 treatment results in a significant decrease of 4-HNE-protein content in the plasma of apoE^–/–mice. Alda-1 administration leads to a slight increase in gene expression related to neurogenesis (Nog), mitochondrial biogenesis (CYTB,ND1), and apoptosis (Bax,Gsk3b) in the Hp of apoE^–/–mice. Alda-1 administration leads to 2 and 10 differentially expressed proteins in the FCx and Hp of apoE^–/–mice, respectively^[1].
Alda-1 (1.5 mg/kg, b.w., IP) administration significantly increases the climbing time, tends to reduce the immobility time and increases the swimming time of the prenatally stressed rats in the forced swim test. Moreover, treatment of prenatally stressed rats with Alda-1 significantly increases number of entries into the open arms of the maze and the time spent therein, as assessed by elevated plus-maze test^[2]. Alda-1 (8.5 mg/kg; IP) with glucose significantly lowers 4-HNE and FJB-positive cells in the cerebral cortex of Alda-1-treated rats than in DMSO-treated rats 24 h after glucose administration^[3].
Alda-1 (10 mg/kg per day) treatment prevents aldehydic overload, mitochondrial dysfunction and improves ventricular function in post-MI cardiomyopathy rats^[4].

324.16

Solid

C₁₅H₁₁Cl₂NO₃

349438-38-6

Room temperature in continental US; may vary elsewhere.

DMSO : ≥ 51 mg/mL(157.33 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 0.5%CMC-Na/saline water

  Solubility: 24 mg/mL (74.04 mM); Suspended solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.5 mg/mL (7.71 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (7.71 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。