Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.

Thioflavin T (ThT) is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3.The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission^[1].

318.86

Solid

C₁₇H₁₉ClN₂S

2390-54-7

硫黄素T

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 16.67 mg/mL(52.28 mM;Need ultrasonic)

H₂O : 12.5 mg/mL(39.20 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

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  Description: Thioflavin T is a fluorescent dye used for staining Aβ fibrils with green fluorescence. Thioflavin T is weakly fluorescent in aqueous solutions but exhibits an enhanced emission at 482 nm upon binding to Aβ plaques. Thioflavin T can be applied to the research of Alzheimer disease.
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  Description: Thioflavin T can be used to detect endoplasmic reticulum stress (ERS) by exhibiting fluorescence when it binds to protein aggregates with green fluorescence.
  Method: For dye binding assay.
  1. Cells are fixed in 4% paraformaldehyde, permeabilized by 0.1% Triton X/PBS, washed for 3 times.
  2. Incubate cells with Thioflavin T (5 μM; 20 min; 37℃).
  3. Wash cells for three times with PBS, mount cells to slides using two drops of Fluoromount and stored at 4℃ in the dark.
  4. Confocal images are captured using an automatic microscope (BX63, Olympus Corp).