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Luteolin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Luteolin图片
CAS NO:491-70-3
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
50mg电议
100mg电议
500mg电议
1 g电议
5 g电议

产品名称
木犀草素
Luteoline
Luteolol
Digitoflavone
产品介绍
Luteolin (Luteoline), 一种黄酮类化合物,也是一种有效的Nrf2抑制剂。Luteolin 具有抗炎和抗癌特性,可在人类多种癌细胞系 (包括非小肺癌细胞) 中诱导细胞凋亡和细胞周期停滞,并抑制细胞转移和血管生成。
生物活性

Luteolin (Luteoline), a flavanoid compound, is a potentNrf2inhibitor. Luteolin has anti-inflammatory, anti-cancer properties, including the induction ofapoptosisand cell cycle arrest, and the inhibition of metastasis and angiogenesis, in severalcancercell lines, including human non-small lungcancercells[1][2][3].

体外研究
(In Vitro)

Luteolin (0-160 μM; 24 hours; NCI-H460 cells) treatment inhibits the viability of NCI-H460 cells in a concentration-dependent manner[1].
Luteolin (20-80 μM; 24 hours; NCI-H460 cells) treatment causes an accumulation of cells in the S phase[1].
Luteolin (320-580 μM; 48 hours; NCI-H460 cells) treatment induces apoptosis[1].
Luteolin (20-80 μM; 24 hours; NCI-H460 cells) treatment increases the protein expression levels of apoptotic regulatory proteins, including the Bax/Bcl-2 ratio, in a concentration-dependent manner, however, only 80 μM Luteolin inhibits the expression of Bad. Luteolin also decreases the expression of Sirt1 in the NCI-H460 cell line in a concentration-dependent manner[1].

Cell Viability Assay[1]

Cell Line:NCI-H460 cells
Concentration:0 μM, 20 μM, 40 μM, 80 μM and 160 μM
Incubation Time:24 hours
Result:Inhibited the viability of NCI-H460 cells in a concentration-dependent manner.

Cell Cycle Analysis[1]

Cell Line:NCI-H460 cells
Concentration:20 μM, 40 μM, 80 μM
Incubation Time:24 hours
Result:Induced cell cycle arrest in the S phase.

Apoptosis Analysis[1]

Cell Line:NCI-H460 cells
Concentration:320 μM, 440 μM, 580 μM
Incubation Time:48 hours
Result:Apoptotic fraction was markedly increased.

Western Blot Analysis[1]

Cell Line:NCI-H460 cells
Concentration:20 μM, 40 μM, 80 μM
Incubation Time:24 hours
Result:Increased the protein expression levels of apoptotic regulatory proteins and decreased the expression of Sirt1 in the NCI-H460 cell line in a concentration-dependent manner.
体内研究
(In Vivo)

Luteolin (10-100 mg/kg; oral gavage; daily; for 12 weeks; adult male Wistar rats) has an antioxidant effect and can also protect against non-alcoholic steatohepatitis through targeting the pro-inflammatory IL-1 and Il-18 pathways in rats with a high carbohydrate/high fat diet[2].

Animal Model:Adult male Wistar rats (200-220 g)[2]
Dosage:10 mg/kg, 25 mg/kg, 50 mg/kg or 100 mg/kg
Administration:Oral gavage; daily; for 12 weeks
Result:Significantly reduced ALT and AST activity and reduced levels of bilirubin, hyaluronic acid and malondialdehyde. Shows an antioxidant activity such as a significant increase in reduced glutathione. IFN-γ, TNF-α, IL-1α and IL-18 levels decreased significantly.
Clinical Trial
分子量

286.24

性状

Solid

Formula

C15H10O6

CAS 号

491-70-3

中文名称

木犀草素;檞片素;木樨草素

结构分类
  • Flavonoids
  • Flavones
  • Phenols
  • Polyphenols
来源
  • Plants
  • other families
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(349.36 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.4936 mL17.4679 mL34.9357 mL
5 mM0.6987 mL3.4936 mL6.9871 mL
10 mM0.3494 mL1.7468 mL3.4936 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (7.27 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (7.27 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (7.27 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。