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Dihydroethidium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Dihydroethidium图片
CAS NO:104821-25-2
包装与价格:
包装价格(元)
10mg电议
50mg电议
100mg电议

产品名称
二氢乙锭
Hydroethidine
PD-MY 003
产品介绍
Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化 (Ex/Em=518/616 nm)。
生物活性

Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red (Ex/Em=518/616 nm).

分子量

315.41

性状

Solid

Formula

C21H21N3

CAS 号

104821-25-2

Emission (Em)
Em 380-450 nm Violet; Em 590-620 nm Orange
Excitation (Ex)
Ex<380 nm Ultraviolet; Ex 380-450 nm Violet; Ex 495-570 nm Green
中文名称

二氢乙锭

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : ≥ 50 mg/mL(158.52 mM)

H2O : 0.67 mg/mL(2.12 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.1705 mL15.8524 mL31.7048 mL
5 mM0.6341 mL3.1705 mL6.3410 mL
10 mM0.3170 mL1.5852 mL3.1705 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.93 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used to detect ROS production.
    Method: For the detection of ROS production in liver tissues.
    1. Frozen sections of liver tissues in each group (5-μm-thick) are prepared and incubated with Dihydroethidium (7.5 mM; 30 min; 37℃; dark).
    2. The sections are observed using a fluorescence microscope (Leica, Germany) and the percentage of Dihydroethidiumpositive cells is calculated through a quantitative morphometric analysis.
  • Description: Dihydroethidium is a superoxide indicator, it can be used to detect superoxide anion production. Dihydroethidium specifically reacts with intracellular superoxide anion and turns into ethidium in nuclei with red fluorescence.
  • Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used for the estimation of reactive oxygen species (ROS) levels.
    Method: For paraffin sections staining.
    1. Paraffin sections (5 μM) are used to measure retinal and choroidal ROS production with Dihydroethidium (5 μmol/L; 20 min; 37℃).
    2. To confirm the specificity of Dihydroethidium staining, eye slides are preincubated with 100 U/mL polyethylene glycol-conjugated superoxide dismutase (30 min; 37℃).
    3. Use an Olympus DP73 fluorescence microscope (Tokyo, Japan) and Image J-NIH freeware to measure the intensity of staining.
  • Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used to indicate the ROS accumulation.
    Method: For brain sections staining.
    1. Incubate brain sections in 10 μM Dihydroethidium solution (1 h; 37℃; dark).
    2. Wash sections with PBS for 3 times.
    3. Samples are mounted with neutral balata and observed with the fluorescent microscope (Carl Zeiss, Weimar, Germany)and the positive area is calculated using Image J.