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Disitertide diammonium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
P144 diammonium
产品介绍
Disitertide (P144) diammonium 是转化生长因子TGFβ1的多肽抑制剂,特异性的阻断其与受体间的相互作用。Disitertide diammonium 也是PI3K的抑制剂和凋亡 (apoptosis) 诱导剂。
生物活性

Disitertide (P144) diammonium is a peptidictransforming growth factor-beta 1 (TGF-β1)inhibitor specifically designed to block the interaction with its receptor. Disitertide diammonium is also aPI3Kinhibitor and anapoptosisinducer[1][2][3][4][5].

体外研究
(In Vitro)

Disitertide (P144, 100 μg/mL) diammonium suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells[2].
Disitertide (TGF-β1 inhibitor) diammonium abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance[3].
Disitertide (10 μg/mL to 200 μg/mL) diammonium affects proliferation, induces apoptosis as well as anoikis in A172 and U-87 MG GBM cell lines[5].

Western Blot Analysis[2]

Cell Line:Mouse embryo osteoblast precursor MC3T3-E1 cells.
Concentration:100 μg/mL
Incubation Time:4 h
Result:Significantly suppressed the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group.
体内研究
(In Vivo)

Disitertide (P144, Topical application, 300 μg/mL) diammonium may promote scar maturation and improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment[4].

Animal Model:Human hypertrophic scars were implanted in 60 nude mice[4].
Dosage:300 μg/mL was added the Lipogel.
Administration:Topical application daily administered.
Result:Successful shedding was achieved in 83.3% of the xenografts.
Clinical Trial
分子量

1614.88

性状

Solid

Formula

C68H115N19O22S2

Sequence Shortening

TSLDASIIWAMMQN

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 10 mg/mL(6.19 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.6192 mL3.0962 mL6.1924 mL
5 mM0.1238 mL0.6192 mL1.2385 mL
10 mM---------
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 50%PEG300   50% saline

    Solubility: 5 mg/mL (3.10 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。