5-Ethynyl-2'-deoxyuridine

立即咨询

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

61135-33-9

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
50mg	电议
100mg	电议
250mg	电议
500mg	电议
1 g	电议
5 g	电议

产品介绍

5-Ethynyl-2'-deoxyuridine (EdU) 是一种胸腺嘧啶核苷类似物，在细胞增殖时能够插入正在复制的 DNA 分子中， EdU 与染料的共轭反应可以进行高效快速的细胞增殖检测分析。5-Ethynyl-2'-deoxyuridine 是一种 PROTAC linker，属于 alkyl chain 类。可用于合成 PROTAC 分子。

生物活性

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation^[1]. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-basedPROTAClinker that can be used in the synthesis ofPROTACs^[2].

IC₅₀& Target

Alkyl-Chain

体外研究
(In Vitro)

5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system^[1].
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

体内研究
(In Vivo)

The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two month old female mice^[1].

Animal Model:	Two month old female mice^[1]
Dosage:	10, 20, 50, 100 or 200 mg/kg
Administration:	Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result:	EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.

分子量

252.22

性状

Solid

Formula

C₁₁H₁₂N₂O₅

CAS 号

61135-33-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 50 mg/mL(198.24 mM;Need ultrasonic)

H₂O : 25 mg/mL(99.12 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.9648 mL	19.8240 mL	39.6479 mL
5 mM	0.7930 mL	3.9648 mL	7.9296 mL
10 mM	0.3965 mL	1.9824 mL	3.9648 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 4 mg/mL (15.86 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。