您好，欢迎来到化工原料网！ [登录] [免费注册]
化工原料网
发布求购
位置：首页 > 产品库 > Lycorine
立即咨询
咨询类型：
     
*姓名：
*电话：
*单位：
Email：
*留言内容：
请详细说明您的需求。
*验证码：
 
Lycorine
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Lycorine图片
CAS NO:476-28-8
包装与价格：
包装价格(元)
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
石蒜碱
产品介绍
Lycorine 是从石蒜科植物中提取的天然生物碱。Lycorine 是强效具有口服活性的SCAP 抑制剂，Kd值 15.24 nM。Lycorine 下调 SCAP 蛋白水平而不改变其转录。Lycorine 也是黑色素瘤血管生成抑制剂。Lycorine 有潜力用于前列腺癌和代谢疾病的研究。
生物活性

Lycorine is a natural alkaloid extracted from theAmaryllidaceaeplant. Lycorine is a potent and orally activeSCAPinhibitor with aKdvalue 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription[2]. Lycorine is also amelanoma vasculogenicinhibitor[3]. Lycorine can be used for the study of prostatecancerand metabolic diseases[2].

IC50& Target

Kd: 15.24 nM (SCAP)[1]

体外研究
(In Vitro)

Lycorine inhibits cell proliferation in a dose-dependent manner in the abovementioned 4 PCa cell lines, and the IC50ranged from 5 μM to 10 μM., it also shows Lycorine has little effects on PNT1A cell's proliferation[1].
SCAP (SREBF chaperone) is an ER-toGolgi transport protein, undergoes a conformational change, and regulates/preserves SREBFs in the ER by forming a complex with INSIG1 (insulin induced gene 1)[2].
Lycorine (5-40 μM; 16 hours) significantly suppresses SREBFs activity (up to -70%) in a dosedependent manner and does not cause obvious cytotoxicity in cells[2].
Lycorine (10-20 μM; 2-16 hours) dose- and time-dependently decreases the mature SREBF1 and SREBF2 proteins in HL-7702 cells[2].
Lycorine (20 μM; 16 hours) neither activates NR1H3 transcription nor affect NR1H3 target genes, including ABCG5 and ABCG8, but Sterols activates NR1H3 transcription activity[1].
Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected[3].
Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion[3].

Cell Viability Assay[2]

Cell Line:HL-7702/SRE-Luc cells
Concentration:16 hours
Incubation Time:5 μM; 10 μM; 20 μM; 40 μM
Result:Had no cytotoxicity on HL-7702 cells.

Western Blot Analysis[2]

Cell Line:HL-7702/SRE-Luc cells
Concentration:2 hours, 4 hours, 8 hours, 12 hours, 16 hours
Incubation Time:10 μM; 20 μM
Result:Decreased p-SREBF1, m-SREBF1, p-SREBF2 and p-SREBF1 protein expression.

RT-PCR[3]

Cell Line:C8161 cells
Concentration:0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:48 hours
Result:Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.
体内研究
(In Vivo)

Lycorine (oral chow; 15 mg/kg, 30 mg/kg; once daily) alleviates fat accumulation and metabolic syndrome, increases lipolysis and betaoxidation of fatty acid along with precursor and mature SREBFs in mice[2].

Animal Model:High-fat diet (HFD)-fed C57BL/6J mice[2]
Dosage:15 mg/kg, 30 mg/kg
Administration:Oral chow; once daily
Result:Ameliorated high-fat diet-induced hyperlipidemia, hepatic steatosis, and insulin resistance in mice.
分子量

287.31

性状

Solid

Formula

C16H17NO4

CAS 号

476-28-8

中文名称

石蒜碱

结构分类
  • Alkaloids
  • Isoquinoline Alkaloids
  • Alkaloids
  • Pyrrole Alkaloids
来源
  • Plants
  • Amaryllidaceae
  • Plants
  • other families
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 25 mg/mL(87.01 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.4806 mL17.4028 mL34.8056 mL
5 mM0.6961 mL3.4806 mL6.9611 mL
10 mM0.3481 mL1.7403 mL3.4806 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。