Lupeol (Clerodol; Monogynol B; Fagarasterol) is an active pentacyclic triterpenoid, has anti-oxidant, anti-mutagenic, anti-tumor and anti-inflammatory activity. Lupeol is a potent androgen receptor(AR) inhibitor and can be used forcancerresearch, especially prostatecancerof androgen-dependent phenotype (ADPC) and castration resistant phenotype (CRPC)^[1].

Androgen receptor^[1]

Lupeol, an effective AR inhibitor, can be developed as a potential agent to treat human prostate cancer (CaP). Lupeol (10–50 μM) treatment for 48 h results in a dose-dependent growth inhibition of androgen-dependent phenotype (ADPC) cells viz., LAPC4 and LNCaP cells with an IC₅₀of 15.9 and 17.3 μM, respectively. Lupeol also inhibits the growth of 22Rν_1 with an IC₅₀of 19.1 μM. Further, Lupeol inhibits the growth of C4-2b cells with an IC₅₀of 25 μM. Lupeol has the potential to inhibit the growth of CaP cells of both ADPC and CRPC phenotype. Androgens by activating AR are known to drive the growth of CaP cells^[1].

Lupeol is an effective agent that has the potential to inhibit the tumorigenicity of CaP cells in vivo. At the conclusion of the study on day 56, the total circulating serum-PSA levels (secreted by the implanted tumor cells) are measured. At 56thday post-implantation, PSA levels are observed between 11.95-12.79 ng/mL in control animals with LNCaP-tumors and C4-2b-tumors, respectively. However, Lupeol-treated counterpart animals exhibits reduced serum-PSA levels in a range of 4.25-7.09 ng/mL. Tumor tissues of animals receiving Lupeol treatment exhibits reduced serum-PSA levels as compared to control^[1].

426.72

Solid

C₃₀H₅₀O

545-47-1

羽扇豆醇；羽扇醇；羽扇豆醇酯；蛇麻醇酯

• Terpenoids
• Triterpenes

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Room temperature in continental US; may vary elsewhere.

Ethanol : 14.29 mg/mL(33.49 mM;ultrasonic and warming and heat to 60℃)

DMSO : 2 mg/mL(4.69 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： corn oil

  Solubility: 20 mg/mL (46.87 mM); Clear solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 10% EtOH    90%corn oil

  Solubility: ≥ 1.43 mg/mL (3.35 mM); Clear solution

  此方案可获得 ≥ 1.43 mg/mL (3.35 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中，混合均匀。