FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages ofapoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of cells (HeLa cells for example)^[1]:
1. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with10 μLof propidium iodide (PI),10 μLof FITC-Dextran (MW 10000) (the final concentration of PI and FITC-Dextran (MW 10000) is7.5 μMand1.13 μM, respectively).
2. Incubate cells for25 minat room temperature in the dark.
3. Take the labeled cells with3 mLof medium and centrifuge for10 minat 500g.
4. Take centrifuged cells with1 mLof medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 10000): Ex=495 nm, Em=525 nm).

10000.00(Average)

Solid

60842-46-8

荧光素异硫氰酸酯-葡聚糖(MW 10000)

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

H₂O : 50 mg/mL(Need ultrasonic)

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (Infinity mM); Clear solution; Need ultrasonic

• 
  Description: FITC-Dextran MW 10000 can be used to explore the cellular uptake with green fluorescence.
  Method: For cellular uptake monitoring.
  1. Incubate cells in glass dishes (37℃; overnight).
  2. Incubate cells with FITC-Dextran MW 10000 (5 μg/mL; 4 h).
  3. Wash cells for twice with PBS and finally fix cells with 4% PFA (30 min; dark).
  4. Use a confocal laser scanning microscopy (Zeiss 880) for image.
• 
  Description: FITC-Dextran MW 10000 can be used to detect intestinal barrier function in vivo.
  Method: For assess intestinal barrier function.
  1. Fast mice for 4 hours.
  2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g).
  3. Measure fluorescence intensity of plasma in 4 hours.
• 
  Description: FITC-Dextran MW 10000 can be used for labeling microvessels in the lesion area of rat brain to measure microvessel density.
  Method: For in vivo labeling.
  1. Anesthetize rats first.
  2. Inject FITC-Dextran MW 10000 (50 mg/kg) in the tail vein of rats.
  3. Remove brain tissues 1 min after injection, use 4% paraformaldehyde (4℃; 24 h) to fix tissues and cut tissues into 150 μm-thick sections.
  4. Use a laser scanning confocal microscope (Leica, Solms, Germany) for image.