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Fluo-4 AM
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Fluo-4 AM图片
CAS NO:273221-67-3
包装:100 μg
市场价:1500元

产品介绍
Fluo-4 AM 是一种常用的检测细胞内 Ca2+浓度的探针。
生物活性

Fluo-4 AM is a cell-permeable Ca2+ indicator[1].

体外研究
(In Vitro)

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Count the cells and take 106 cells from each sample (control and experiment/s).
2. Collect the cells (5 min, 3000×g, 4 ℃) and wash once in PBS.
3. Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 ℃ for 1 h.
4. Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.
5. Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.

分子量

1096.94

性状

Solid

Formula

C51H50F2N2O23

CAS 号

273221-67-3

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex 495-570 nm Green
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

染色示例
  • Description: Fluo-4 AM can be used to determine total intracellular Ca2+levels.
    Method: For cell labeling.
    1. Count cells and take 106cells from each sample.
    2. Collect cells (5 min, 3000×g, 4℃) and wash once in PBS.
    3. Resuspend cells in 0.5 mL PBS and add Fluo-4 AM (1 mM; 0.5 μL; 37℃; 1 h) to a final concentration of 1 μM.
    4. Wash cells three times (2 min, 3000×g) with PBS and finally resuspend in 1 mL PBS, and separated into 2 flow cytometry tubes-0.5 mL in each.
    5. Evaluate staining by flow cytometry and analyze the data by a software such as CellQuest software.