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5-Carboxyfluorescein diacetate N-succinimidyl ester
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
5-Carboxyfluorescein diacetate N-succinimidyl ester图片
CAS NO:150206-05-6
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品名称
5-羧基二乙酸荧光素 N-琥珀酰亚胺酯
产品介绍
5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm,Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合,从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。
生物活性

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of5 μM) and mix rapidly.
3. After5 minat room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 ℃.
5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 ℃, and remove the supernatant.
Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.

分子量

557.46

性状

Solid

Formula

C29H19NO11

CAS 号

150206-05-6

中文名称

5-羧基二乙酸荧光素 N-琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 20.83 mg/mL(37.37 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.7939 mL8.9693 mL17.9385 mL
5 mM0.3588 mL1.7939 mL3.5877 mL
10 mM0.1794 mL0.8969 mL1.7939 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。