Pembrolizumab (MK-3475) 是一种人源化 IgG4 抗体,为PD-1的抑制剂,用于癌症免疫的研究。
生物活性 | Pembrolizumab (MK-3475) is a humanizedIgG4antibody inhibiting the programmed cell death 1 (PD-1) receptor, used incancer immunotherapy. |
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体外研究 (In Vitro) | Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells[2]. Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells[2].
Cell Cytotoxicity Assay[2] Cell Line: | αROR1-CAR T and A549 cells | Concentration: | 10 μg/mL | Incubation Time: | Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days. | Result: | Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure. |
Apoptosis Analysis[2] Cell Line: | A549 cells | Concentration: | 10 μg/mL | Incubation Time: | | Result: | Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity. |
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体内研究 (In Vivo) | Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure[2].
Animal Model: | Six-week-old male BALB/c athymic nude mice[2] | Dosage: | 5 mg/kg | Administration: | Pembrolizumab (5 mg/kg; Intravenous injection; Once) | Result: | Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |