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Pembrolizumab
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:1374853-91-4
包装与价格:
包装价格(元)
1mg电议
5mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品名称
MK-3475
Lambrolizumab
产品介绍
Pembrolizumab (MK-3475) 是一种人源化 IgG4 抗体,为PD-1的抑制剂,用于癌症免疫的研究。
生物活性

Pembrolizumab (MK-3475) is a humanizedIgG4antibody inhibiting the programmed cell death 1 (PD-1) receptor, used incancer immunotherapy.

IC50& Target

IC50: 0.6 nM (PD-1)

体外研究
(In Vitro)

Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells[2].
Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells[2].

Cell Cytotoxicity Assay[2]

Cell Line:αROR1-CAR T and A549 cells
Concentration:10 μg/mL
Incubation Time:Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result:Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis[2]

Cell Line:A549 cells
Concentration:10 μg/mL
Incubation Time:
Result:Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.
体内研究
(In Vivo)

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure[2].

Animal Model:Six-week-old male BALB/c athymic nude mice[2]
Dosage:5 mg/kg
Administration:Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.
Clinical Trial
分子量

146266.23

性状

Liquid

CAS 号

1374853-91-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.