Biotin (Vitamin B7) 是一种水溶性维生素 B，是人体五种羧化酶的辅酶，参与脂肪酸、异亮氨酸和缬氨酸的合成，并参与糖异生。Biotin 是细胞生长、脂肪酸产生以及脂肪和氨基酸代谢的必需物质。
| 生物活性 | Biotin (Vitamin B7) is a water-soluble B vitamin and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats andamino acids[1][2][3]. |
| IC50& Target | Microbial Metabolite | Human Endogenous Metabolite |
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体外研究
(In Vitro) | Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively[4].
Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmaxof 27.34 pmol/mg protein/min[4].
Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 μM)-induced cell death and reduces cell adhesion[5].
Cell Viability Assay[5] | Cell Line: | Murine oligodendrocyte 158N cells | | Concentration: | 1, 10, 100, 1000 nM | | Incubation Time: | 24 hours | | Result: | Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status.
Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration. |
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体内研究
(In Vivo) | Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice[6].
Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose[7].
| Animal Model: | Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)[6] | | Dosage: | 15 mg/kg/d | | Administration: | Oral gavage; 12 days | | Result: | Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage. |
| Animal Model: | Grass carp (117±0.5 g)[7] | | Dosage: | 0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg | | Administration: | Oral gavage; 70 days | | Result: | Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM).
Reduced the mRNA levels of anti-microbial substances.
Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(409.32 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.0932 mL | 20.4658 mL | 40.9316 mL | | 5 mM | 0.8186 mL | 4.0932 mL | 8.1863 mL | | 10 mM | 0.4093 mL | 2.0466 mL | 4.0932 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (10.23 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (10.23 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (10.23 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 4. 请依序添加每种溶剂： PBS Solubility: 1.96 mg/mL (8.02 mM); Clear solution; Need ultrasonic and warming and heat to 60℃ *以上所有助溶剂都可在本网站选购。
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