Capsaicin ((E)-Capsaicin) 是辣椒中的活性成分，是一种TRPV1激动剂。Capsaicin 具有减轻疼痛、抗氧化、抗炎、神经保护和抗癌作用。
| 生物活性 | Capsaicin ((E)-Capsaicin), an active component of chili peppers, is aTRPV1agonist. Capsaicin has pain relief, antioxidant, anti-inflammatory, neuroprotection and anti-cancer effects[1][2]. |
| IC50& Target | EC50: 290 nM (hTRPV1, in HEK293 cell)[1] |
体外研究
(In Vitro) | Capsaicin (50-300 μM; 24-72 hours) shows an augmented decrease in cell growth in a dose- and time-dependent manner. The observed IC50 value is around 150 μM[2].
Capsaicin (50-300 μM; 24-72 hours) shows increase in cytosolic cytochrome c, activation of caspase 3 and PARP (p85) levels, and decreases anti-apoptotic Bcl-2 protein and increases pro-apoptotic Bad/Bax expression[2].
Capsaicin increases the nuclear condensation, nuclear DNA fragmentation and sub-G1 DNA content[2].
Capsaicin suppresses the cell cycle progression at the G1/S phase in FaDu cells by decreasing the expression of the regulators of cyclin B1 and D1, as well as cyclin-dependent protein kinases cdk-1, cdk-2 and cdk-4[2].
Cell Viability Assay[2] | Cell Line: | Human pharyngeal squamous carcinoma cells (FaDu) cells | | Concentration: | 50 μM, 100 μM, 200 μM, and 300 μM | | Incubation Time: | 24 hours, 48 hours and 72 hours | | Result: | Showed an augmented decrease in cell growth. |
Apoptosis Analysis[2] | Cell Line: | FaDu cells | | Concentration: | 50 μM, 100 μM and 200 μM | | Incubation Time: | 12 hours | | Result: | Increased the activity of caspase 3 in a time-dependent manner. |
Western Blot Analysis[2] | Cell Line: | FaDu cells | | Concentration: | 200 μM | | Incubation Time: | 24 hours | | Result: | The observed activation of caspase 3 and PARP (p85) levels. |
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体内研究
(In Vivo) | Capsaicin suppresses the development of lung carcinoma by amending the protein expressions of apoptotic regulators p53, Bcl-2, Bax and caspase-3[2].
| Animal Model: | Swiss albino mice (20-25 g; 8-10 weeks old) induced by Benzo(a)pyrene[3] | | Dosage: | 10 mg/kg | | Administration: | Intraperitoneal administration; once in a week; for 14 weeks | | Result: | Inhibited the development of mice lung carcinogenesis. |
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| 来源 | - Plants
- Solanaceae
- Capsicum annuumL.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(327.43 mM;Need ultrasonic) Ethanol : 100 mg/mL(327.43 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.2743 mL | 16.3714 mL | 32.7429 mL | | 5 mM | 0.6549 mL | 3.2743 mL | 6.5486 mL | | 10 mM | 0.3274 mL | 1.6371 mL | 3.2743 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% EtOH 90%corn oil Solubility: ≥ 20 mg/mL (65.49 mM); Clear solution
此方案可获得 ≥ 20 mg/mL (65.49 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 200.0 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中，混合均匀。 2. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.19 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.19 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 4. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.19 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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