Indimitecan (LMP776) 是一种拓扑异构酶 I (Top1) 抑制剂,具有抗癌活性。
生物活性 | Indimitecan (LMP776) is atopoisomeraseI (Top1) inhibitor with anticancer activities[1]. |
IC50& Target | |
体外研究 (In Vitro) | Indimitecan (LMP776) (0-100 μM) shows antiproliferative potencies against various human cancer cell lines, with mean-graph midpoint (MGM) of 0.079 ± 0.023 μM[1]. Indimitecan shows potent DNA cleavage due to Top1 inhibition[1]. Indimitecan can be substrates for metabolic ketone reductases[1].
Cell Proliferation Assay[1] Cell Line: | HOP-62, HCT-116, SF-539, UACC-62, OVCAR-3, SN12C, DU-145, MCF-7 | Concentration: | 0-100 μM | Incubation Time: | | Result: | Showed growth inhibition with GI50s of<0.01,<0.01, 0.04,<0.01, 0.08,<0.01,<0.01 and 0.01 μM against HOP-62, HCT-116, SF-539, UACC-62, OVCAR-3, SN12C, DU-145 and MCF-7 cells, respectively. And the mean-graph midpoint (MGM) for growth inhibition of all human cancer cell lines successfully tested (the National Cancer Institute’s developmental therapeutics assay (the “NCI60”)) was 0.079 ± 0.023 μM. |
|
Clinical Trial | |
分子量 | |
性状 | |
Formula | |
CAS 号 | |
运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
|
溶解性数据 | In Vitro: DMSO : 8.33 mg/mL(18.13 mM;ultrasonic and warming and adjust pH to 3 with HCl and heat to 60℃) 配制储备液 1 mM | 2.1765 mL | 10.8826 mL | 21.7652 mL | 5 mM | 0.4353 mL | 2.1765 mL | 4.3530 mL | 10 mM | 0.2177 mL | 1.0883 mL | 2.1765 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 |