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Ciprofloxacin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ciprofloxacin图片
CAS NO:85721-33-1
包装与价格:
包装价格(元)
500mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
环丙沙星
Bay-09867
产品介绍
Ciprofloxacin (Bay-09867) 是一种口服有效的拓扑异构酶 IV 抑制剂。Ciprofloxacin 诱导线粒体 DNA 和核 DNA 损伤并导致线粒体功能障碍和活性氧产生。Ciprofloxacin 具有抗增殖活性并诱导细胞凋亡 (apoptosis)。Ciprofloxacin 是一种氟喹诺酮类抗生素,具有强大的抗菌活性。
生物活性

Ciprofloxacin (Bay-09867) is a potent, orally activetopoisomeraseIVinhibitor. Ciprofloxacin induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin has anti-proliferative activity and inducesapoptosis. Ciprofloxacin is a fluoroquinoloneantibiotic, exhibiting potentantibacterialactivity[1][2][3][4].

IC50& Target

Quinolone

 

体外研究
(In Vitro)

Ciprofloxacin (Bay-09867) (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase[1].
Ciprofloxacin (Bay-09867) shows potent activity againstY. pestisandB. anthraciswith MIC90of 0.03 μg/mL and 0.12 μg/mL, respectively[2].

Cell Cycle Analysis[1]

Cell Line:Tendon cells
Concentration:5, 10, 20 and 50 μg/mL
Incubation Time:24 hours
Result:Decreased the cellularity of tendon cells.

Apoptosis Analysis[1]

Cell Line:Tendon cells
Concentration:50 μg/mL
Incubation Time:24 hours
Result:Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells.

Western Blot Analysis[1]

Cell Line:Tendon cells
Concentration:50 μg/mL
Incubation Time:0, 6, 12, 17 and 24 hours
Result:Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulated the expression of PLK-1 protein.
体内研究
(In Vivo)

Ciprofloxacin (Bay-09867) (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection againstY. pestisin murine model of pneumonic plague[3].
Ciprofloxacin (Bay-09867) (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall[4].
Ciprofloxacin (Bay-09867) (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall[4].

Animal Model:BALB/c mice[3]
Dosage:30 mg/kg
Administration:Intraperitoneal injection; for 24 hours
Result:Reduced the lung bacterial load in murine model of pneumonic plague.
Animal Model:C57BL/6J mice[4]
Dosage:100 mg/kg
Administration:Oral gavage; daily, for 4 weeks
Result:Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity.
Animal Model:C57BL/6J mice[4]
Dosage:100 mg/kg
Administration:Oral gavage; daily, for 4 weeks
Result:Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.
Clinical Trial
分子量

331.34

性状

Solid

Formula

C17H18FN3O3

CAS 号

85721-33-1

中文名称

环丙沙星

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

0.1 M HCL : 16.67 mg/mL(50.31 mM;ultrasonic and warming and adjust pH to 2 with HCl and heat to 60℃)

H2O :< 0.1 mg/mL(insoluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.0180 mL15.0902 mL30.1805 mL
5 mM0.6036 mL3.0180 mL6.0361 mL
10 mM0.3018 mL1.5090 mL3.0180 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。