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4-Methylumbelliferyl-β-D-Glucopyranoside
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
4-Methylumbelliferyl-β-D-Glucopyranoside图片
CAS NO:18997-57-4
包装与价格：
包装价格(元)
10mM (in 1mL DMSO)电议
1g电议
5g电议

产品介绍
4-甲基伞形酮基-β；-D-吡喃葡萄糖苷，一种 β；-D-葡萄糖苷，是 β；-葡萄糖苷酶的荧光底物，用于测定 β；-葡萄糖苷酶的活性。
Cas No.18997-57-4
别名4-甲基伞形酮酰-Β-D-吡喃葡糖酸苷,4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU
化学名4-methyl-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2H-chromen-2-one
Canonical SMILESOC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O1
分子式C16H18O8
分子量338.3
溶解度≥ 84.5mg/mL in DMSO with gentle warming
储存条件Store at 2-8°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease.1,2,3Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4

References:

1. Hernández-Guzmán, A., Flores-Martínez, A., Ponce-Noyola, P., et al. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii FEBS Open Bio. 6(11), 1067-1077 (2016).
2. Kwapiszewski, R., Skolimowski, M., Ziólkowska, K., et al. A microfluidic device with fluorimetric detection for intracellular components analysis Biomed Microdevices 13(3), 431-440 (2011).
3. Shanmuganathan, M.K., and Britz-McKibbin, P. Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis Anal. Bioanal. Chem. 399(8), 2843-2853 (2011).
4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule J. Spectrosc. 1(1), 147128 (2013).