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tcY-NH2 TFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
tcY-NH2 TFA图片
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
(trans-Cinnamoyl)-YPGKF-NH2 TFA
产品介绍
tcY-NH2 ((trans-Cinnamoyl)-YPGKF-NH2) TFA 是一种有效的PAR4选择性肽类拮抗剂。tcY-NH2 TFA 抑制凝血酶和 AY-NH2诱导的血小板聚集和内皮抑素释放,可用于炎症、免疫学的研究。
生物活性

tcY-NH2 ((trans-Cinnamoyl)-YPGKF-NH2) TFA is a potent selectivePAR4antagonist peptide. tcY-NH2 TFA inhibits thrombin- and AY-NH2-induced platelet aggregation and endostatin release, and can be used in the research of inflammation, immunology[1][2][6].

体外研究
(In Vitro)

tcY-NH2 TFA (0-500 μM) inhibits AYPGKF-NH2(10 μM)-induced platelet (obtained from male albino Sprague–Dawley rats) aggregation, with an IC50value of 95 μM[1].
tcY-NH2 TFA potently activates aorta relaxation (RA) and gastric (LM) contraction, with IC50values of 64 μM (RA) and 1 μM (LM)[1].
tcY-NH2 TFA (Tc-YPGKF-NH2, 400 μM, 5 min) prevents endostatin release and platelet aggregation induced by thrombin or by AY-NH2[2].
tcY-NH2 TFA (5 μM, 15 min) decreases infarct size (IS) by 51%, and increases recovery of ventricular function by 26% in an isolated heart model[5].

体内研究
(In Vivo)

tcY-NH2 TFA (tail vein injection, 0.6 mg/kg for a single dose) alleviates liver injury in Brain death (BD) rat model, indicated by lower serum ALT/AST levels and better histomorphology[3].
tcY-NH2 TFA (intraperitoneal injection, 0.6 mg/kg for a single dose) increases posttraumatic activation of CD4+Tregs within the draining lymph nodes in burn injury mice model[4].
tcY-NH2 TFA (intrapleural injection, 40 ng/kg for a single dose) inhibits neutrophil recruitment in experimental inflammation in mice[6].

Animal Model:Brain death (BD) rat model[3]
Dosage:0.6 mg/kg for a single dose
Administration:Tail vein injection for a single dose
Result:Reduced blood platelet activation and hepatic platelet accumulation.
Attenuated the inflammatory response and apoptosis in the livers.
Inhibited the activation of NF-κB and MAPK pathways induced by Brain death (BD).
Animal Model:Burn injury model of C57BL/6 N mice[4]
Dosage:0.6 mg/kg for a single dose
Administration:Intraperitoneal injection
Result:Increased expression and phosphorylation of PKC-θ in the presence of platelets, without affecting early posttraumatic hemostasis.
Animal Model:BALB/c mice[6]
Dosage:40 ng/kg for a single dose
Administration:Intrapleural injection
Result:Abolished the number of rolling and adhering neutrophils on the vessel wall.
Inhibited CXCL8- and Cg-induced neutrophil migration into the pleural cavity of mice.
分子量

853.88

性状

Solid

Formula

C42H50F3N7O9

Sequence Shortening

{trans-Cinnamoyl}-YPGKF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(117.11 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.1711 mL5.8556 mL11.7112 mL
5 mM0.2342 mL1.1711 mL2.3422 mL
10 mM0.1171 mL0.5856 mL1.1711 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (2.44 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.44 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (2.44 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.44 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (2.44 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.44 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。