L-NAME hydrochloride

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本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

51298-62-5

包装与价格：

包装	价格(元)
10 mM * 1 mL in Water	电议
100mg	电议
500mg	电议
1 g	电议
5 g	电议

产品名称

NG-Nitroarginine methyl ester hydrochloride

产品介绍

L-NAME hydrochloride是NOS的抑制剂，IC₅₀为70 μM。

生物活性

L-NAME hydrochloride inhibitsNOSwith anIC₅₀of 70 μM. L-NAME is a precursor toNOSinhibitor L-NOARG which has anIC₅₀value of 1.4 μM.

IC₅₀& Target

IC50: 70 μM (NOS)^[1]

体外研究
(In Vitro)

L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with N^w-nitro-L-arginine methyl ester (L-NAME) being at the head^[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC₅₀= 70 μM) than L-NOARG (IC₅₀= 1.4 μM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG^[1].

体内研究
(In Vivo)

L-NAME infusion significantly decreases NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and IFNγ production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes^[3]. There is increasing evidence that nitric oxide may be involved in learning and memory. l-NAME produces a task-dependent impairment of fear extinction, and implies that nitric oxide signaling is involved in memory process of certain fear extinction tasks^[4]. Chronic L-NAME administration induces cardiac hypertrophy in rodent models. Six weeks L-NAME administration induces significant cardiac hypertrophy compared to control hearts^[5].

Clinical Trial

分子量

269.69

性状

Solid

Formula

C₇H₁₆ClN₅O₄

CAS 号

51298-62-5

中文名称

N-硝基-L-精氨酸甲酯；N'-硝基-L-精氨酸甲酯盐酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(370.80 mM;Need ultrasonic)

H₂O : 100 mg/mL(370.80 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.7080 mL	18.5398 mL	37.0796 mL
5 mM	0.7416 mL	3.7080 mL	7.4159 mL
10 mM	0.3708 mL	1.8540 mL	3.7080 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 140 mg/mL (519.11 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。