4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside 是 N-乙酰基-β-D-氨基葡萄糖苷酶的荧光底物。
| Cas No. | 37067-30-4 |
| 别名 | 4-甲基香豆素-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷 |
| Canonical SMILES | O=C(C)N[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O |
| 分子式 | C18H21NO8 |
| 分子量 | 379.4 |
| 溶解度 | DMF: 5 mg/ml,DMSO: 30 mg/ml |
| 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for β-hexosaminidases. Upon enzymatic cleavage by β-hexosaminidases, 4-methylumbelliferone (4-μU) is released and its fluorescence can be used to quantify β-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses such as Tay-Sachs disease, which is characterized by defects in the α subunit of β-hexosaminidase. |
m.cnreagent.com