Choline chloride 是一种必需营养素，可激活 α7 烟碱受体 (alpha7 nicotinic receptors)，具有镇痛、抗炎活性。可影响肝脏疾病、动脉粥样硬化和神经系统疾病等疾病。
| 生物活性 | Choline chloride is an essential nutrient that activatesalpha7 nicotinic receptorsand has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders[1][2]. |
| IC50& Target | Human Endogenous Metabolite |
|
体外研究
(In Vitro) | Choline chloride (0 or 70 μM, 4 days) can effectively mitigate apoptosis and maintain cell viability[1].
Cell Viability Assay[1] | Cell Line: | Rat pheochromocytoma cells PC12 | | Concentration: | 0 or 70 μM | | Incubation Time: | 4 days | | Result: | Showed the cell viability of 94% at 70 μM while cell viability of 83% at 0 μM.
Reduced the number of cells with DNA breaks (characteristic of apoptosis) by 8.5% at 70 μM compared to the no treatment group. |
|
体内研究
(In Vivo) | Choline chloride (s.c., 0.2 and 100 mg/kg/h, 24 or 48 hours) can reduce postoperative injurious reflexes and effectively decreases tumor necrosis factor (TNF) release from macrophages in female C57/Bl6 mice[2].
| Animal Model: | Postoperative pain model of female C57/Bl6 mice[2] | | Dosage: | 0.2 and 100 mg/kg/h | | Administration: | Subcutaneous injection, 24 or 48 hours | | Result: | Reduced heat hypersensitivity after surgery with maximal efficacy after 48 h treatment and the ED50value of choline dose was 1.7 mg/kg/h.
Reduced hypersensitivity to punctate mechanical stimuli 48 hours after infusion in a dose-dependent manner and with the ED50value of 4.7 mg/kg/h but not 24 hours. |
|
| Clinical Trial | |
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| 中文名称 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: DMSO : ≥ 140 mg/mL(1002.72 mM) H2O : ≥ 100 mg/mL(716.23 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 7.1623 mL | 35.8115 mL | 71.6230 mL | | 5 mM | 1.4325 mL | 7.1623 mL | 14.3246 mL | | 10 mM | 0.7162 mL | 3.5811 mL | 7.1623 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 130 mg/mL (931.10 mM); Clear solution; Need ultrasonic 2. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution
此方案可获得 ≥ 3.5 mg/mL (25.07 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution
此方案可获得 ≥ 3.5 mg/mL (25.07 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 4. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution
此方案可获得 ≥ 3.5 mg/mL (25.07 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
|
m.cnreagent.com