ML-SA1 作为一种选择性TRPML激动剂，通过促进溶酶体的酸化和蛋白酶活性，抑制登格病毒 2 (DENV2) 和寨卡病毒 (ZIKV)。ML-SA1 抗 DENV2 RNA 和 ZIKV RNA 的IC50值分别为 8.3 μM 和 52.99 μM。ML-SA1 诱发自噬。ML-SA1可用于广谱抗病毒研究。
| 生物活性 | ML-SA1, as a selectiveTRPMLagonist, inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. TheIC50value of ML-SA1 against DENV2 RNA and ZIKV RNA is 8.3 μM and 52.99 μM, respectively. ML-SA1 inducesautophagy. ML-SA1 can be used for the research of broad-spectrum antiviral[1]. |
| IC50& Target | IC50: 8.3 μM (DENV2)[1].
IC50: 52.99 μM (ZIKV)[1] |
体外研究
(In Vitro) | ML-SA1 (25 μM; 0~14 hours; A549 cells) possibly affects the entry of DENV2 into host cells[1].
ML-SA1 (0~200 μM; A549 cells) shows that there is no cytotoxicity to the cell line observed, even at concentrations up to 200 μM. ML-SA1 (0~50 μM; A549 cells) significantly suppresses DENV2 at the RNA levels and the IC50is 8.93 μM[1].
ML-SA1 results in a dose-dependent decrease in ZIKV in A549 cells at both the RNA and protein levels, and the IC50value of ML-SA1 against ZIKV RNA is 52.99 μM. ML-SA1, as an activator of TRPMLs, appears to be a potent inhibitor of DENV2 and ZIKV in vitro. ML-SA1 promotes lysosomal acidification and protease activity to inhibit viral infection. ML-SA1 can induce autophagy in Huh7 cells or A549 cells[1].
Western Blot Analysis[1] | Cell Line: | A549 cells | | Concentration: | 25 μM | | Incubation Time: | 0~14 hours | | Result: | Possibly affected the entry of DENV2 into host cells. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 20.83 mg/mL(57.47 mM;ultrasonic and warming and heat to 65℃) 配制储备液 | 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | | 5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | | 10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.74 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.74 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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