您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Xanthatin
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Xanthatin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Xanthatin图片
CAS NO:26791-73-1
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
苍耳亭
产品介绍
Xanthatin 从Xanthium strumarium叶子中提取,诱导细胞凋亡 (apoptosis)。Xanthatin 通过抑制PGE2的合成和 5-脂氧合酶的活性而显示出抗炎活性。Xanthatin 抑制布鲁氏菌的IC50值为 2.63 μg/ mL,对寄生虫特异性锥虫硫磷还原酶具有不可逆的弱抑制作用。
生物活性

Xanthatin is isolated from Xanthium strumarium leaves. Xanthatin exhibits strong antitumor activities against a variety ofcancercells throughapoptosis persuasion and shows anti-inflammatory activities by inhibiting PGE2 synthesisand 5-lipoxygenase activity[1]. Xanthatin is a potent and orally active inhibitor ofVEGFR2 kinaseactivity with anIC50of 3.8 μM and prominently blocks the phosphorylation of VEGFR2 at Tyr951 site. Xanthatin inhibits angiogenesis and has the potential for the investigation of breastcancer[2].

IC50& Target

IC50: apoptosis; 3.8 μM (VEGFR2 kinase); 2.63 μg/mL (T. b. brucei)[1]

体外研究
(In Vitro)

Xanthatin is against T. b. brucei with an IC50 value of 2.63 μg/mL and exhibits weak irreversible inhibition of parasite specific trypanothione reductase[1].
Xanthatin (0-40 μM; 24 hours) has obscure inhibition effect on the proliferation of HUVEC in the absence of VEGF[2].
Xanthatin (5-40 μM; 24 hours) inhibits breast cancer cell proliferation in a dose responsive manner. Xanthatin inhibits HCC1937, MDA-MB-415, SK-BR-3, MCF-7 and MDA-MB-231 with IC50values of 81 μM, 31 μM, 38 μM, 30 μM, and 17 μM, respectively[2].
Xanthatin (0-10 μM; 24 hours) dose dependently suppresses the phosphorylation of STAT3 (Ser727), at the same time, it also results in a rapid dephosphorylation of down-stream kinases of STAT3, including PI3K and Akt, including PI3K (p-PI3K p85tyr458) and Akt[2].

Cell Proliferation Assay[2]

Cell Line:HUVEC cells
Concentration:0 μM, 5 μM, 10 μM, 15 μM, 20 μM, 30 μM, 40 μM
Incubation Time:24 hours
Result:Inhibited cell growth from dose 10 μM in the presence of vEGF.

Cell Viability Assay[2]

Cell Line:HCC1937, MDA-MB-415, SK-BR-3, MCF-7 and MDA-MB-231 cells
Concentration:5, 10, 15, 20, 30, and 40 μM
Incubation Time:24 hours
Result:Inhibited breast cancer cell growth.

Western Blot Analysis[2]

Cell Line:HUVEC cells
Concentration:0, 3, and 10 μM
Incubation Time:24 hours
Result:Inhibited VEGFR2 downstream signaling pathways and blocked VEGF-induced STAT3 activation in HUVEC.
体内研究
(In Vivo)

Xanthatin (intragastric administration; 20 mg/kg; once daily; 25 days) leads to significant inhibition of tumor volume. And this compound is well-tolerated and exhibits no significant difference in weight compares to the vehicle group[2].

Animal Model:Transplanted MDA-MB-231 cells into mice and constucted human breast cancer xenograft mouse model[2]
Dosage:20 mg/kg; once daily; 25 days
Administration:Intragastric administration
Result:Supressed tumor growth and tumor angiogenesis in vivo.
分子量

246.30

性状

Solid

Formula

C15H18O3

CAS 号

26791-73-1

中文名称

苍耳亭

结构分类
  • Terpenoids
  • Sesquiterpenes
来源
  • Plants
  • Leguminosae
  • Pterocarpus soyauxiiTaub.
  • Plants
  • Compositae
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(406.01 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM4.0601 mL20.3004 mL40.6009 mL
5 mM0.8120 mL4.0601 mL8.1202 mL
10 mM0.4060 mL2.0300 mL4.0601 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 4.55 mg/mL (18.47 mM); Clear solution

    此方案可获得 ≥ 4.55 mg/mL (18.47 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 45.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (10.15 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (10.15 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。