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AGN 194310
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
AGN 194310图片
CAS NO:229961-45-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
1mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品名称
VTP-194310
产品介绍
AGN 194310 (VTP-194310) 是一种高亲和力的、有效的、具有选择性的视黄酸受体 (RARs) 的泛拮抗剂,对RARαRARβRARγKd值分别为 3 nM,2 nM,5 nM。
生物活性

AGN 194310 (VTP-194310) is a high affinity, potent and selectiveretinioic acid receptors (RARs)pan-antagonist withKdvalues of 3 nM, 2 nM, 5 nM forRARα,RARβ,RARγ, respectively[1][2].

体外研究
(In Vitro)

AGN194310 potently inhibits colony formation by all three lines, with IC50 values of 16 nM for LNCaP cells; 18 nM for PC3 cells; and 34 nM for DU-145 cells[2].
AGN 194310 (50 nM, 100 nM; LNCaP, PC-3 and DU-145 cells) inhibits colony formation at concentrations of 50 nM and 100 nM alone and in combination with TTNPB[2].
AGN 194310 (1 μM; 72 hours; LNCaP cells) treatment results in 80% apoptosis[2].

Cell Viability Assay[2]

Cell Line:LNCaP, PC-3 and DU-145 cells.
Concentration:50 nM, 100 nM
Incubation Time:
Result:When used together with 100 nM TTNPB, there was almost complete reversal of the growth inhibitory effect of 50 nM and partial reversal of the effect of 100 nM.

Apoptosis Analysis[2]

Cell Line:LNCaP cells
Concentration:1 μM
Incubation Time:72 hours
Result:Induced apoptosis in LNCaP cells.
体内研究
(In Vivo)

AGN 194310 (0.5 mg/kg/day; oral gavage; every day; for 10 days; female C57Bl/6J mice) treatment increases the number of granulocytes across haemopoietic compartments. A significant increase in the frequency of granulocyte-progenitor cells is observed in the bone marrow of mice after treatment with AGN194310[3].

Animal Model:Female C57Bl/6J mice (Five-week-old (34-37 days))[3]
Dosage:0.5 mg/kg/day
Administration:Oral gavage; every day; for 10 days
Result:The number of granulocytes was significantly increased across haemopoietic compartments. Progenitor cells containing granulocytes also increased significantly.
分子量

424.55

性状

Solid

Formula

C28H24O2S

CAS 号

229961-45-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL(117.77 mM;ultrasonic and warming and heat to 60℃)

H2O :< 0.1 mg/mL(insoluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.3554 mL11.7772 mL23.5544 mL
5 mM0.4711 mL2.3554 mL4.7109 mL
10 mM0.2355 mL1.1777 mL2.3554 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 1 mg/mL (2.36 mM); Clear solution

    此方案可获得 ≥ 1 mg/mL (2.36 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 1 mg/mL (2.36 mM); Clear solution

    此方案可获得 ≥ 1 mg/mL (2.36 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。