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Isatin(Indoline-2,3-dione)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Isatin(Indoline-2,3-dione)图片
CAS NO:91-56-5
规格:≥98%
包装与价格:
包装价格(元)
2g电议
5g电议
10g电议
25g电议
50g电议
100g电议
200g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)147.13
FormulaC8H5NO2
CAS No.91-56-5
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 29 mg/mL (197.1 mM)
Water: <1 mg/mL
Ethanol: 10 mg/mL (68 mM)
Solubility (In vivo)

Chemical Name: indoline-2,3-dione

InChi Key: JXDYKVIHCLTXOP-UHFFFAOYSA-N

InChi Code: InChI=1S/C8H5NO2/c10-7-5-3-1-2-4-6(5)9-8(7)11/h1-4H,(H,9,10,11)

SMILES Code: O=C1NC2=C(C=CC=C2)C1=O

Synonyms2,3-Indolinedione ;Isatin; 2,3-Dioxo-2,3-dihydroindole; Isatic acid lactam; Isotin
实验参考方法
In Vitro

In vitro activity: It has been repoted that isatin treatment at 1-400 μM for 24h could induce a significant dose-dependent increase in MTT metabolism by SH-SY5Y cells, which was not due to the increase in cell division. In addition, isatin at the higher concentrations was able to trigger cell death, though MTT metabolism was still increased, indicating that the surviving cells were hypermetabolic. With a longer treatment, isatin was found to cause cell death in a dose-dependent manner, and the predominant mode of cell death was apoptosis at lower concentrations, while at the highest concentration increasing numbers of necrotic cells were also observed


Cell Assay: Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.

In VivoIsatin is an endogenous indole that is increased in stress, inhibits monoamine oxidase (MAO) B and improves bradykinesia and striatal dopamine levels in rat models of Parkinson's disease. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum. In rodent models isatin has been shown to cause a widespectrum of dose-dependent physiological and biological actions, such as anxiogenic and sedative effects, memory dysfunction and inhibition of food and water intake. Significantly, isatin readily crosses the blood-brain barrier so that a peritoneal dose of 100 mg/kg would result in a concentration of about 120 μM in the rat brain. This concentration would increase further with repeated injections.
Animal modelAlbino rats of the Fisher strain
Formulation & DosageDissolved in DMSO; 100 mg/kg; i.p.
References

Neurochem Int. 2005 Aug;47(3):216-24; Neurotoxicology. 2004 Jan;25(1-2):205-13.