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Salvigenin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Salvigenin图片
CAS NO:19103-54-9
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
三裂鼠尾草素
产品介绍
Salvigenin 是一种天然的多酚类化合物,具有神经保护作用。Salvigenin 具有抗肿瘤、细胞毒和免疫调节作用。Salvigenin 抑制H2O2诱导的细胞凋亡(apoptosis
生物活性

Salvigenin is a natural polyphenolic compound, with neuroprotective effect. Salvigenin has antitumor cytotoxic and immunomodulatory properties. Salvigenin inhibits H2O2-induced cellapoptosis[1][2].

体外研究
(In Vitro)

Salvigenin (0-100 μM, 3 h) protects SH-SY5Y cells against H2O2-induced cell death[1].
Salvigenin (0-100 μM, 2 h) significantly reduces ROS generation, increases the glutathione level, declines the extent of cell apoptosis and increases autophagy in H2O2-exposed SH-SY5Y cells[1].

Cell Viability Assay[1]

Cell Line:SY5Y cells exposed to H2O2(300 μM)
Concentration:10, 25, 50, and 100 μM
Incubation Time:3 h
Result:Protected SH-SY5Y cells (best in 25 and 50μM) against H2O2-induced cell death.

Apoptosis Analysis[1]

Cell Line:SY5Y cells exposed to H2O2(300 μM)
Concentration:10, 25, 50, and 100 μM
Incubation Time:2 h
Result:Significantly declined the extent of cell apoptosis induced by H2O2.

Western Blot Analysis[1]

Cell Line:SY5Y cells exposed to H2O2(300 μM)
Concentration:10, 25, 50, and 100 μM
Incubation Time:2 h
Result:Reduced cleaved caspase-3 and Bax/Bcl-2 ratio. Reduced caspase-12 and calpain levels. Reduced the level of Atg7, Atg12, and LC3-II/ LC3-I.
体内研究
(In Vivo)

Salvigenin (0-9.68 μg/mouse/day; i.p.; 4 or 12 days) shows antitumor and immunomodulatory effects on tumor bearing mice[2].

Animal Model:MCF-7 cell bearing inbred female Balb/c mice, aged from six to eight weeks[2]
Dosage:3.65, 5.85 and 9.68 μg/mouse/day for four days or 12 days
Administration:Intraperitoneal injection
Result:Showed a significant increase in DTH response in a dose-dependent manner. Significantly decreased the rate of tumor growth, increased lymphocyte proliferation index, increased the level of IFN-γ and decreased IL-4 production. Exhibited a significant decrease of the splenic CD4+CD25+Foxp3+T lymphocytes.
分子量

328.32

性状

Solid

Formula

C18H16O6

CAS 号

19103-54-9

中文名称

三裂鼠尾草素

结构分类
  • Flavonoids
  • Flavones
  • Phenols
  • Monophenols
来源
  • Plants
  • other families
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 20 mg/mL(60.92 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.0458 mL15.2290 mL30.4581 mL
5 mM0.6092 mL3.0458 mL6.0916 mL
10 mM0.3046 mL1.5229 mL3.0458 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: 2 mg/mL (6.09 mM); Suspended solution; Need ultrasonic

    此方案可获得 2 mg/mL (6.09 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。