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A 419259 trihydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
A 419259 trihydrochloride图片
CAS NO:1435934-25-0
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议

产品名称
RK 20449 trihydrochloride
产品介绍
A 419259 trihydrochloride是Src家族激酶抑制剂,对SrcLckLynIC50分别为9 nM,3 nM,3 nM。
生物活性

A 419259 trihydrochloride is aSrcfamily kinasesinhibitor withIC50s of 9 nM, 3 nM and 3 nM forSrc,LckandLyn, respectively.

IC50& Target

IC50: 9 nM (Src),<3 nm (lck),<3 (lyn), 3 μm (abl)[1]

体外研究
(In Vitro)

A 419259 Trihydrochloride is a second-generation pyrrolo-pyrimidine designed to enhance selectivity towards the Src family relative to other cytoplasmic tyrosine kinases. A-419259 inhibits K-562 cells with an IC50between 0.1 and 0.3 μM, and Meg-01 proliferation with an IC50of approximately 0.1 μM. A-419259 also potently induces apoptosis in K-562 cells beginning at 0.1 μM and increasing in a dose-dependent manner. PP2 inhibits Src kinase autophosphorylation in both Ph+cell lines (K-562 and Meg-01) with an IC50between 3 and 10 μM, while A-419259 blocks kinase activation between 0.1 and 0.3 μM. A-419259 strongly inhibits DAGM/Bcr-Abl cell proliferation in the absence of IL-3 with an IC50between 0.1 and 0.3 μM[1]. A-419259 is a broad-spectrum pyrrolo-pyrimidine inhibitor, and blocks proliferation and induces apoptosis in CML cell lines. A-419259 inhibits overall SFK activity in K562 and other CML cell lines with an IC50value of 0.1-0.3 μM[2].

分子量

592.00

性状

Solid

Formula

C29H37Cl3N6O

CAS 号

1435934-25-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : ≥ 55 mg/mL(92.91 mM)

DMSO : 1 mg/mL(1.69 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.6892 mL8.4459 mL16.8919 mL
5 mM0.3378 mL1.6892 mL3.3784 mL
10 mM0.1689 mL0.8446 mL1.6892 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (168.92 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。