β-Carotene (Provitamin A) 属于类胡萝卜素化合物，是天然存在的维生素 A 前体。β-Carotene 是一种ROS调节剂，具有抗氧化和抗炎活性。β-Carotene 可作为抗氧化剂或者促氧化剂，取决于它的内在特性以及它所作用的生物环境的氧化还原电位。
β-Carotene 可诱导乳腺癌细胞凋亡 (apoptosis)，具有抗癌活性。
| 生物活性 | β-Carotene (Provitamin A), a carotenoid compound, is a naturally-occurring vitamin A precursor. β-Carotene is a modulator ofreactive oxygen species(ROS), with antioxidant and antiinflammatory activities. β-Carotene may serve as an antioxidant or as a prooxidant, depending on its intrinsic properties as well as on the redox potential of the biological environment in which it acts. β-Carotene induces breastcancercellsapoptosis, with anticancer activities[1][2][3][4][5]. |
| IC50& Target[3] | Human Endogenous Metabolite | apoptosis |
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体外研究
(In Vitro) | β-Carotene up-regulatesPPAR-γexpression andROSproduction in MCF-7 cancer cells[3].
β-Carotene (1-100 μM; 72 hours) remarkably decreases the survival of MCF-7 cells in a dose-dependent manner[3].
β-Carotene (50 μM; 24-72 hours) significantly enhances the expression levels of PPAR-γ mRNA and protein in a time-dependent manner[3].
β-Carotene down-regulates the COX-2 but up-regulates the p21 mRNA level and protein expression in a time dependent manner[3].
β-Carotene significantly increases the percentage of early apoptosis and the effect was partly attenuated by pre-incubation with GW9662 (HY-16578) or GSH (HY-D0187)[3].
β-Carotene induces cytochrome C release[3].
Cell Viability Assay[5] | Cell Line: | MCF-7 cells | | Concentration: | 1 μM, 10 μM, 20 μM, 50 μM, 100 μM | | Incubation Time: | 72 hours | | Result: | Decreased the numbers of viable cells to 70% and 50% at 20 μM and 50 μM, respectively. |
RT-PCR[5] | Cell Line: | MCF-7 cells | | Concentration: | 50 μM | | Incubation Time: | 24 hours, 48 hours, 72 hours | | Result: | Up-regulated the PPAR-γ mRNA. |
Western Blot Analysis[5] | Cell Line: | MCF-7 cells | | Concentration: | 50 μM | | Incubation Time: | 24 hours, 48 hours, 72 hours | | Result: | Up-regulated PPAR-γ protein expression levels. |
Apoptosis Analysis[5] | Cell Line: | MCF-7 cells | | Concentration: | 50 μM | | Incubation Time: | 72 hours | | Result: | Induced MCF-7 cells apoptosis. |
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| 结构分类 | - Terpenoids
- Other Terpenoids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
| 溶解性数据 | In Vitro: THF : 12.5 mg/mL(23.28 mM;ultrasonic and warming and heat to 60℃) Acetone : 5 mg/mL(9.31 mM;Need ultrasonic) DMSO : 1 mg/mL(1.86 mM;ultrasonic and warming and heat to 60℃) 配制储备液 | 1 mM | 1.8626 mL | 9.3132 mL | 18.6265 mL | | 5 mM | 0.3725 mL | 1.8626 mL | 3.7253 mL | | 10 mM | 0.1863 mL | 0.9313 mL | 1.8626 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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