Fast-TRFS 是硫氧还蛋白还原酶 (TrxR) 的选择性超快荧光探针。Fast-TRFS 可用于对活细胞中的 TrxR 活性进行成像。
生物活性 | Fast-TRFS is a selective and superfast fluorogenic probe ofthioredoxin reductase (TrxR). Fast-TRFS can be used for imagingTrxRactivity in live cells[1]. |
体外研究 (In Vitro) | Fast-TRFS reaches the maximal fluorescence signal within 1 min incubated with TCEP, and within 5 min incubated with the TrxR enzyme[1]. Fast-TRFS (10 μM; 2-60 min) appears the blue fluorescence signal within 2 min in HeLa cells[1].
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: DMSO : 100 mg/mL(265.70 mM;Need ultrasonic) 配制储备液 1 mM | 2.6570 mL | 13.2848 mL | 26.5696 mL | 5 mM | 0.5314 mL | 2.6570 mL | 5.3139 mL | 10 mM | 0.2657 mL | 1.3285 mL | 2.6570 mL |
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以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: 2.5 mg/mL (6.64 mM); Suspended solution; Need ultrasonic
此方案可获得 2.5 mg/mL (6.64 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
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染色示例 | Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.Method: For determine location of Fast-TRFS inE.coli.1. To detect Fast-TRFS, mid-log phaseE.colibacteria are treated with the probe for 5 min.2. Then, bacterial cells, at concentrations of 106colony forming units (CFU)/mL, are transferred to slides for observation under the confocal microscope (Nikon, Japan). Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.Method: For Fast-TRFS assay.1. 10 μM of Fast-TRFS is added to both untreated and TrxR inhibitors treated bacteria.2. The TRFS fluorescence intensity is measured using a multimode reader (M200 Tecan, Austria). Images of cells are taken under inverted fluorescent microscope (Nikon, japan).
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