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Fast-TRFS
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Fast-TRFS图片
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
Fast-TRFS 是硫氧还蛋白还原酶 (TrxR) 的选择性超快荧光探针。Fast-TRFS 可用于对活细胞中的 TrxR 活性进行成像。
生物活性

Fast-TRFS is a selective and superfast fluorogenic probe ofthioredoxin reductase (TrxR). Fast-TRFS can be used for imagingTrxRactivity in live cells[1].

体外研究
(In Vitro)

Fast-TRFS reaches the maximal fluorescence signal within 1 min incubated with TCEP, and within 5 min incubated with the TrxR enzyme[1].
Fast-TRFS (10 μM; 2-60 min) appears the blue fluorescence signal within 2 min in HeLa cells[1].

分子量

376.37

性状

Solid

Formula

C14H11F3N2O3S2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(265.70 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.6570 mL13.2848 mL26.5696 mL
5 mM0.5314 mL2.6570 mL5.3139 mL
10 mM0.2657 mL1.3285 mL2.6570 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: 2.5 mg/mL (6.64 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (6.64 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.
    Method: For determine location of Fast-TRFS inE.coli.
    1. To detect Fast-TRFS, mid-log phaseE.colibacteria are treated with the probe for 5 min.
    2. Then, bacterial cells, at concentrations of 106colony forming units (CFU)/mL, are transferred to slides for observation under the confocal microscope (Nikon, Japan).
  • Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.
    Method: For Fast-TRFS assay.
    1. 10 μM of Fast-TRFS is added to both untreated and TrxR inhibitors treated bacteria.
    2. The TRFS fluorescence intensity is measured using a multimode reader (M200 Tecan, Austria). Images of cells are taken under inverted fluorescent microscope (Nikon, japan).