Diphenylterazine (DTZ) 是一种生物发光剂。Diphenylterazine 本身仅产生很少的背景，导致极好的信噪比。
| 生物活性 | Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios[1]. |
体外研究
(In Vitro) | Diphenylterazine elicits minimal cell toxicity at millimolar concentrations[1].
Notes: To make a stock solution for DTZ (Diphenylterazine), first, a premixture is prepared by dissolving 17.6 mg of L-ascorbic acid (HY-B0166) in 10 mL ethanol and 10 mL 1,2-propanediol; next, 1 mg of DTZ is dissolved in 88 μL of the premix, resulting in a 30 mM DTZ stock solution containing 5 mM L-ascorbic acid[3].
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体内研究
(In Vivo) | Diphenylterazine injections into untransfected BALB/c mice do not yield any background emission. The bioluminescence resulting from intraperitoneally injected Diphenylterazine displays extended kinetics[1].
DTZ (0.3 μmol/mouse (1.13 mg.ml-1/100 ul/mouse); i.v.) treatment can track tumor growth in a xenograft NU/J mouse model[2].
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -80°C, protect from light, stored under nitrogen |
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(264.94 mM;Need ultrasonic;DMSO can inactivate Diphenylterazine's activity) DMF : 11.11 mg/mL(29.44 mM;Need ultrasonic;DMSO can inactivate Diphenylterazine's activity) EtOH+HCl : 1 mg/mL(2.65 mM;ultrasonic and warming and adjust pH to 2 with 1M HCl and heat to 80℃;DMSO can inactivate Diphenylterazine's activity) H2O :< 0.1 mg/mL(insoluble;DMSO can inactivate Diphenylterazine's activity) 配制储备液 | 1 mM | 2.6494 mL | 13.2471 mL | 26.4943 mL | | 5 mM | 0.5299 mL | 2.6494 mL | 5.2989 mL | | 10 mM | 0.2649 mL | 1.3247 mL | 2.6494 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 50%PEG300 50% saline Solubility: 2 mg/mL (5.30 mM); Suspended solution; Need ultrasonic 2. 请依序添加每种溶剂： 10% DMF 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 1.11 mg/mL (2.94 mM); Clear solution
*以上所有助溶剂都可在本网站选购。 |
| 染色示例 | Description: Diphenylterazine (DTZ) can be used as a bioluminescence agent for bioluminescent imaging in vitro and in vivo.Method: For vivo use.1. Anesthetized animals.2. Animals are anesthetized again after the first anesthetized for 36 hours and apple saline containing Diphenylterazine to the eye surface of animals (for eye studies). After anesthetized for 48 hours, then inject Diphenylterazine solution into both the left leg and the right leg by intraperitoneal injection (for skeletal muscle studies). Description: Diphenylterazine can be used as an optimal substrate for Antares2.Method: For cell labeling.1. Add Diphenylterazine (500, 50, 5, and 0.5 μM) into the culture media containing PC3/CD63-Antares2 cells.2. Use a bioluminescence imaging system for image. Description: Diphenylterazine can be used to track tumor growth in vivo.Method: For vivo use.1. Diphenylterazine (0.3 μM; intravenous injection) is injected into testing animals.2. Use an in vivo imaging system for image.
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