Dansylcadaverine (Monodansyl cadaverine) 是一种用于标记自噬空泡的自荧光化合物。Dansylcadaverine 是谷氨酰胺转胺酶的高亲和力底物，能阻断受体介导的多种配体的内吞作用。
| 生物活性 | Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands[1][2]. |
体外研究
(In Vitro) | The inhibitory activity of dansylcadaverine reflects its ability to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules[2].
Dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid A, and is displaced competitively by other compounds which possess affinity toward endotoxins[3].
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
| 溶解性数据 | In Vitro: DMSO : 62.5 mg/mL(186.31 mM;Need ultrasonic) H2O : 1 mg/mL(2.98 mM;ultrasonic and warming and heat to 80℃) 配制储备液 | 1 mM | 2.9810 mL | 14.9049 mL | 29.8098 mL | | 5 mM | 0.5962 mL | 2.9810 mL | 5.9620 mL | | 10 mM | 0.2981 mL | 1.4905 mL | 2.9810 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
| 染色示例 | Description: Dansylcadaverine is an acid dye with green fluorescence, it is a specific marker for autophagic vacuoles, and it can be used for the detection and quantification of autophagy.Method: For Dansylcadaverine staining.1. Cells are seeded in 6-well plates at a concentration of 3×104cells/well in a 5% CO2incubator at 37℃ for 24 h.2. Following transfection, 50 mM Dansylcadaverine is added to each well for 15 min incubation, and then rinse cells for 3 times with PBS.3. The fluorescence is visualized under a confocal laser-scanning microscope (TCS 4D; Leica, Heidelberg, Germany). Description: Dansylcadaverine is an acid dye with green fluorescence, it can be used as a specific autophagolysosome marker to analyze the autophagic process.Method: For Dansylcadaverine staining.1. Cells are cultured on confocal dishes at a density of 1×104cells/mL overnight and then added with testing compounds for one hour.2. After stimulation with RANKL (50 ng/mL) for five days, cells are incubated with Dansylcadaverine (50 mM; 15 min; 37℃).3. Wash cells with 1×PBS for three times with 5 min interval.4. Finally, the cells are observed under a confocal laser scanning microscopy.
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