BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog ofcholesterolwith a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)^[1].

BODIPY-cholesterol assay
To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM^[1].
Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration^[1].
Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 μM), mixed thoroughly and incubated for 10 -20 mins at 37 ℃^[1].
The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment^[1].

576.61

Solid

C₃₆H₅₁BF₂N₂O

878557-19-8

Room temperature in continental US; may vary elsewhere.

DMSO : 1 mg/mL(1.73 mM;Need ultrasonic and warming)

H₂O :< 0.1 mg/mL (ultrasonic;warming;heat to 60℃)(insoluble)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

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  Description: BODIPY-Cholesterol can be used as a fluorescent monitor for sterol uptake and inter-organelle sterol flux in cells.
  Method: For cell staining.
  1. Stain cells with BODIPY-Cholesterol (1 μM; 20 min; 37℃).
  2. Use a microscope for observation.